+1443 776-2705 panelessays@gmail.com

Write briefly on the following environmental Forensics tools

1. Aerial and Satellite Photography

2. Age dating releases

3. Identification of sources and pathways

4. Remote Sensing Multi-spectral Surveys

5. Identification of ecological stresses and stressors

6. Contaminant identification

7. Summary distribution of contaminants

8. Laboratory Analysis

9. Source identification and types

N.B: For environmental Scientists or those in similar fields. Find attached copies of the course notes for assistance and input.

Environmental Forensics

Babatunde Bolaji Benard

[email protected]

The Overview of Pollution in the Niger Delta

Air pollution

Major causes

Industries

Automobiles

Other activities that release carbon monoxide into the atmosphere

Effects

Human health

Soots

Animals

Plants

The atmosphere

Artisanal refining sites and equipment

What is Environmental Forensic

Environmental forensics has emerged as an important area of environmental studies over the past two decades.

There are two basic aspects to any environmental investigation.

The first being a conventional approach where the standard EPA (Environmental Protection Agency) methods are used to determine concentrations of selected compounds released into the environment. These methods are extremely well documented and widely used, but only provide information on specific target compounds. Whilst this information may be useful for monitoring purposes it is of little use when trying to determine the source of a spill or contaminants in the environment.

If the purpose of an investigation is to determine the source of a contaminant, or point of release, then it is necessary to use a wide variety of analytical techniques and integrate all of the resulting data into one comprehensive data set. It may not always be possible to obtain a unique answer, particularly in the case of groundwater contaminants where there might only be one compound, for example PAH. In that case if there are multiple possible sources in the area it may be difficult to narrow it down to a specific source. Furthermore fingerprinting tools that may be useful with complex mixtures may not be directly applicable to single component mixtures.

Historical Perspective

Application

Sources of Pollution

Fate of Contaminants

Contaminant Source Tracking (Food Chain)

Tools

Aerial and satellite Photography

Age dating releases

• Identification of sources and pathways

Remote Sensing Multi-spectral Surveys

• Identify ecological stresses and stressors

• Contaminant identification

• Summary distribution of contaminants

Laboratory Analysis

Identification

Source identification

Cost allocation Cost allocation

Types

GC with various detectors GC with various detectors

Spectrophotometric at various wavelengths Spectrophotometric at various wavelengths

Radiochemistry

Electrochemical

Microscopy

Crystallography

Physical and chemical properties

Toxicological

Tools Cont’d

Products of forensic Investigations

Unique Identifiers

Congener analysis

Biomarkers

Isotopic ratios

Additives

Fingerprinting

Speciation

Current Bacterial indicators of Fecal Contamination

Total coliforms:

drinking, bathing and shellfish water standards

not feces-specific (environmental sources).

Fecal (“thermotolerant”) coliforms (FC):

detect by growing at elevated temperature of 44-45oC

ditto total coliforms, but less so

E. coli: the “fecal” coliform

Detect and distinguish from other total and fecal coliforms by Beta-glucuronidase activity

may occur naturally in tropical environments (and possibly elsewhere)

Fecal streptococci (FS):

Mostly Lancefield group D (and some group Q) streptococci and enterococci

not feces-specific.

Enterococci:

More feces-specific sub-set of FS: Enterococcus faecalis & E. faecium

EPA guideline for bathing water quality

Microbial Source Tracking (MST)

MST techniques can be divided into two categories:

1. Molecular (genotypic) and biochemical (phenotypic) techniques rely on the close association of certain microorganisms (generally bacteria or viruses), with a specific host, and genetic or phenotypic differences that allow host-specific microbes to be discriminated from others.

2. Chemical methods generally rely on the detection of chemicals associated with anthropogenic activities.

Molecular and Biochemical Techniques

Molecular and biochemical MST techniques can be divided into two broad categories: library dependent and library-independent.

• Library-dependent techniques identify fecal sources from water samples based on a library or database of bacteria isolated from known fecal sources. The library is developed by collecting microbial isolates from known potential sources. The molecular or biochemical pattern of the individual microbial isolates is sometimes referred to as a fingerprint. These identifying patterns can be discerned by a variety of methods, e.g., ribotyping (molecular) and antibiotic resistance analysis (biochemical).

– Molecular (genotypic) techniques are based on the genetic makeup of a cell or organism, e.g., ribotyping, pulsed field gel electrophoresis, rep- polymerase chain reaction (PCR).

– Biochemical (phenotypic) techniques use observable characteristics or traits of an organism such as biochemical or physiological properties, e.g., antibiotic resistance analysis, carbon source utilization.

Library-Dependent Techniques (LDT)

A library is a database of fingerprints from individual bacterial isolates, obtained from potential pollution sources. Bacteria for a source library are normally recovered from animal feaces, though bacteria from animal waste lagoons, septic tanks, and wastewater treatment plants can be used. Most library-dependent techniques require a cultivation step to obtain the bacterial isolates that will be used to generate the library (knowns) and the water bacterial isolates (unknowns) that will be compared against the library.

Library-dependent techniques rely on either molecular or biochemical discrimination of isolates. A number of different microbes may be used for library-dependent techniques including feacal coliforms, Escherichia coli (E. coli), Enterococcus species (enterococci), or feacal streptococci (essentially enterococci, but includes several additional Enterococcus species).

Recently the trend in MST research has been to move away from library-based techniques. This is due in part to their performance in the Southern California Coastal Water Research Project comparison study (SCCWRP) (SCCWRP, 2003; Hagedorn et al., 2011). In addition, the need to develop large site-specific libraries has decreased the interest in using library-dependent approaches (Santo Domingo et al., 2011).

(LDT)

Molecular (Genotypic) (LDT)

Molecular techniques are based directly on the genetic material of the bacterial or viral organism. Bacteria used for these techniques are usually E. coli or Enterococcus spp.

The theory behind these MST techniques is that unique strains of a bacteria species are adapted to their known specific environment (intestines of a particular host species) and, as a result, differ genetically from other strains found in other host species.

A number of genotypic methods are used to type bacteria for library-dependent techniques including ribotyping, pulse field gel electrophoresis (PFGE), and repetitive (rep) polymerase chain reaction (PCR). These techniques are described below.

It is important to note that (1) the genotypic techniques described below differ in discriminatory ability, and (2) bacterial isolates that are grouped into the same “strain” by one method may be separated into distinct strains by a more discriminatory method. The Bibliography section of this report includes studies that have used these techniques.

Molecular (Genotypic) (LDT) Cont’d

Molecular (Genotypic) (LDT) Cont’d

Ribotyping:

Ribotyping has been one of the most widely used techniques in library-dependent MST applications (EPA, 2011). Ribotyping is based on the detection of genetic differences in the genomic sequences within or flanking the 16S and 23S ribosomal ribonucleic acid (rRNA) genes. These rRNA genes are highly conserved in bacteria (EPA, 2005).

For this method, the chosen bacterial group is cultured from fecal samples using standard techniques. E. coli or enterococci are isolated, and a few isolates are picked for genotypic characterization (generally a percentage of the bacterial count). Genomic DNA is isolated for each E. coli strain. Bacterial DNA is digested into fragments using restriction enzymes.

DNA fragments are separated by size using gel electrophoresis. The fragments are transferred to a gel blot, and a labeled probe is used to hybridize to certain portions of the rRNA genes. Because the genome contains several copies of the rRNA genes dispersed throughout the chromosome, the binding of the probe to the DNA fragments which contain it creates a banding pattern that can be visualized by autoradiography or chemical development. These patterns can be used to discriminate among bacterial strains

Ribotyping Cont’d

The banding pattern is captured using digital cameras. Difference in the size and location of the banding patterns can then be compared to known sources in the library database. Image analysis to compare banding patterns can be performed using commercially available software (Scott et al., 2002; Rees et al., 2010). Variables in ribotyping include the type of fecal indicator bacteria used to form the library, as well as the type and number of restriction enzymes used to fragment the DNA. It has been suggested that two restriction enzymes should be routinely used to increase the technique’s discriminatory ability (EPA, 2005).

Advantages This method can be used to classify isolates from multiple sources. When performed by a skilled technician, it is highly reproducible.

Disadvantages Ribotyping is a demanding procedure that requires multiple steps and specialized equipment.

Also, the need for specialized training, high supply costs, and the time required to complete the procedure are disadvantages. As with many genotypic techniques, lab-to-lab variation, issues of repeatability, gel variability, and analysis techniques often make comparison of results from different laboratories difficult.

Complex statistical analysis is often required to determine which sources are likely present. A good working knowledge of statistics is needed. The database (library) size, geographic distribution of isolated bacteria, and the presence of replicate isolates in the bacterial source library affect the ability of ribotyping to differentiate among bacteria at the host-species level (EPA, 2005). In addition, both genotypic and phenotypic techniques would likely break down in complex watersheds with numerous sources (Rees et al., 2010; Harwood, 2011).

Ribotyping Cont’d

Molecular (Genotypic) (LDT) Cont’d

Pulsed Field Gel Electrophoresis (PFGE)

One of the most common techniques, PFGE, is similar to ribotyping. The difference is the whole DNA genome is used instead of the rRNA portion of the genome. Initial steps for obtaining bacterial isolates are the same as ribotyping.

PFGE uses infrequently cutting restriction enzymes on the entire DNA genome. The procedure for DNA isolation is crucial, as large genomic fragments are generated which must not be broken during sample preparation. The genomic fragments are then separated by alternately pulsed, perpendicularly oriented electrical fields, instead of using standard gel electrophoresis. After electrophoresis and staining of the gels, a banding pattern emerges. Patterns are compared to known sources in the library database.

Pulsed Field Gel Electrophoresis (PFGE) Cont’d

Advantages PFGE can be used to classify isolates from multiple sources, and it is among the most discriminatory genotyping methods. When performed by a skilled technician, the method is highly reproducible.

Disadvantages PFGE requires a high degree of technical skill and specific equipment, is time consuming, and is relatively expensive (EPA, 2011). As with ribotyping, a large, geographically-specific source database (library) is required.

Complex statistical analysis is often required to determine which sources are likely present. A good working knowledge of statistics is needed. Both genotypic and phenotypic techniques would likely break down in complex watersheds with numerous sources (Rees et al., 2010; Harwood, 2011).

Repetitive Palindromic Polymerase Chain Reactions (rep-PCR)

PCR allows for rapid amplification of target DNA sequences. PCR is used both in cultivation dependent and independent approaches. For the rep-PCR technique, intervening sequences between certain repetitive portions of the microbial DNA are amplified using rep-PCR and one primer that targets each end of the repetitive, palindromic sequence.

Repetitive DNA elements are scattered throughout the bacterial genome and are separated by distances which vary according to the bacterial species or strain, which forms the basis for the discriminatory patterns generated by rep-PCR. BOX-PCR is a variant of rep-PCR that uses a different primer in the PCR step.

The amplified DNA fragments are separated in agarose gels, producing a banding pattern or “fingerprint” that discriminates among bacterial strains. Bacteria having the same pattern are considered to be of the same strain.

Advantages

Rep-PCR can be used to classify isolates from multiple sources. Compared to the other library techniques, rep-PCR is quicker, easier to use, less expensive, and potentially has a faster turnaround time.

Disadvantages

Although relatively simple compared to PFGE and ribotyping, rep-PCR results tend to be somewhat less reproducible than PFGE or ribotyping (EPA, 2011).

A highly trained technician is required to obtain reproducible results. Complex statistical analysis is often required to determine which sources are likely present.

A good working knowledge of statistics is needed.

As with the other library techniques, a large source database (library) is required that is geographically specific.

In addition, both genotypic and phenotypic techniques would likely break down in complex watersheds with numerous sources (Rees et al., 2010; Harwood, 2011).

Biochemical (Phenotypic) (LDT)

Biochemical techniques are based on observable physical or biochemical characteristics of an organism, as determined by both genetic information and environmental influences.

Librarydependent biochemical techniques include antibiotic resistance analysis and carbon and nutrient utilization profiling. The Bibliography section includes studies that have used these techniques.

Biochemical (Phenotypic) (LDT) Cont’d

Antibiotic Resistance Analysis (ARA)

ARA uses patterns of antibiotic resistance for identifying sources of fecal contamination. The premise is that humans and animals are exposed to different types of antibiotics, and that this selective pressure will alter the antibiotic resistance profile of their fecal bacteria.

These differences should be useful in discriminating among fecal bacterial sources. For this method to be applied, a source library must be developed, using fecal samples from potential contributors in the watershed (e.g., human, livestock, wildlife).

The known sources are analyzed for antibiotic resistance and patterns of resistance. Discriminant analysis (a form of multiple analysis of variance) or logistic regression (a model used to predict the probability of an occurrence) uses the antibiotic resistance patterns from known sources to generate the predictive equations that are used to classify unknown isolates by source.

Antibiotic Resistance Analysis (ARA)

Advantages ARA is relatively simple and fast, requiring less technical expertise and expensive equipment than genotypic methods. These techniques can distinguish multiple sources including human and domestic animals.

Disadvantages Complex statistical analysis is often required to determine which sources are likely present. A good working knowledge of statistics is needed. A geographically-specific reference database is required because phenotypic techniques are geographic and temporally specific. In addition, both genotypic and phenotypic techniques would likely break down in complex watersheds with numerous sources (Rees et al., 2010; Harwood, 2011).

Biochemical (Phenotypic) (LDT) Cont’d

Carbon Utilization Profile (CUP) and Nutrient Utilization Pattern (NUP).

Both CUP and NUP are based on differences among bacterial uses of a wide range of carbon and nitrogen sources for energy and growth. For CUP and NUP, the BIOLOG system allows the user to rapidly perform, score, and tabulate 96 carbon or nitrogen source utilization tests per isolate. Like ARA, the patterns of known sources can be analyzed using discriminant analysis to generate predictive equations that are used to classify unknown isolates using a source library.

While CUP and NUP work well in the laboratory for pure culture characterization/identification, there are many environmental factors in a watershed that can affect bacterial nutrient requirements that may make this method impractical for field determination (Simpson et al., 2002). Like ARA, the CUP method is relatively simple and allows for the analysis of hundreds of isolates in a short period of time.

Carbon Utilization Profile (CUP) and Nutrient Utilization Pattern (NUP) Cont’d.

Advantages CUP is relatively simple and fast, requiring very little technical expertise. Equipment and supplies are expensive (Harwood, 2011). These techniques can distinguish multiple domestic animal sources. However, the CUP and NUP techniques have been tested on a small scale and therefore require more testing.

Disadvantages Complex statistical analysis is often required to determine which sources are likely present. A good working knowledge of statistics is needed. A geographically-specific reference database is required because phenotypic techniques are geographic and temporally specific. In addition, both genotypic and phenotypic techniques would likely break down in complex watersheds with numerous sources (Rees et al., 2010; Harwood, 2011).

Library-independent techniques do not require the development of a source library database.

These techniques rely on a species-specific genotype or characteristic. A variety of bacteria and viruses have been used for MST. It is important to consider the MST indicator survival rate and abundance in the environment. Survival of microbial indicators depends on a variety of factors including their physiology, exposure to radiation, temperature, salinity, predation and competition, amount of organic matter present, and the type of sediments available (Harwood, 2011). Some indicators more closely correlate with fecal indicator bacteria (e.g., E. coli and fecal coliforms).

Dr Babatunde Bolaji Benard

Environmental Forensics

Environmental Forensics

  • What is “Environmental Forensics”?
  • “Environmental Forensics” can be defined as a scientific methodology developed for identifying petroleum-related and other potentially hazardous environmental contaminants and for determining their sources and time of release. It combines experimental analytical procedures with scientific principles derived from the disciplines of organic geochemistry and hydrogeology. Environmental Forensics provides a valuable tool for obtaining scientifically proven, court admissible evidence in environmental legal disputes.
  • Much of the information required in this approach will not be obtained from the data obtained using the conventional EPA methods

*

Basic Environmental Forensic Questions

  • What is the product?
  • Is there more than one source and, if so, which one caused the problem?
  • How long has it been there?
  • Is it degrading?

RITS Fall 2008: Utilization of Stable Isotopes…

*

With any forensic environmental study the basic questions that must be asked are summarized here. First what is it? A second question how many sources and if more than one which one (s) is causing the problem. Then how long has it been there-the reason for this is basically did you own the site when the spill occurred. If it can be shown the age of the spill is such that it occurred before you owned the site that is very advantageous. Finally is the product degrading? This becomes important for cleanup processes. Can you demonstrate natural attenuation is occurring-if so then that is very advantageous for clean up strategies.

Crude Oils and Related Products

*

Fingerprinting and Correlation

  • What are the most commonly used techniques for such purposes?

Gas chromatography

Mass Spectrometry

Gas chromatography-Isotope Ratio Mass Spectrometry (GCIRMS)

Minutes

GC Fingerprints of Different Products

Gasoline

Condensate

JP4

Diesel

0

7

14

21

28

35

42

49

56

63

70

0

7

14

21

28

35

42

49

56

63

70

Minutes

0

7

14

21

28

35

42

49

56

63

70

Minutes

0

7

14

21

28

35

42

49

56

63

70

Minutes

GC Fingerprints of different products

  • Although GC permits product identification, many gasoline samples, for example, will be chromatographically similar, even if from different sources.
  • Refined products generally do not contain biomarkers making GCMS of little consequence.
  • If refined products are from different sources, stable isotopes may provide a potential solution.

Crude Oil Chromatogram

0

C17

C35

Pristane

Phytane

Biomarker Distributions

Utilization of Stable Isotopes

  • What is the product? NO
  • Is there more than one source and, if so, which one caused the problem? YES
  • How long has it been there? NO
  • Is it degrading? YES

RITS Fall 2008: Utilization of Stable Isotopes…

*

This is basically indicating which questions listed in the previous slide can be addressed through the use of stable isotopes.

Why do compounds derived from different feedstocks have different isotope values?

Utilization of Stable Isotopes

*

Carbon Isotopes

  • Carbon in fossil fuels is initially derived from atmospheric CO2. During photosynthesis, fractionation of the two isotopes occurs with preferential assimilation of the lighter isotopes.

*

Carbon Isotopes

  • Extent of fractionation during photosynthesis depends on factors such as: plant type; marine v. terrigenous; C3 v. C4 plant types; temperature; sunlight intensity; water depth.
  • (C3Temperate plants; trees; not grasses; 95% plant species -22 to -30; C4 plants grasses; sugar cane; corn; higher temps and sunlight-10 to -14 per mil)

*

Stable Isotope Determinations

ISOTOPIC VALUES CAN BE MEASURED IN TWO WAYS:

  • BULK ISOTOPES
  • ISOTOPIC COMPOSITIONS OF INDIVIDUAL COMPOUNDS

Isotope Values of Crude Oils Vary with Source

*

-20

-25

-30

-35

-23.27

-25.66

-30.06

-29.52

-29.50

-23.45

-29.68

Monterey Crude

Katalla Crude

Cook Inlet Crude

North Slope Crude

Unknown Source

Monterey Source

NSC Source

Petroleum Source

d13C

Correlations Using Carbon Isotopes

*

d13C of Aromatics (‰, PDB)

d13C of Saturates (‰, PDB)

BP “American Trader” Accident 5/7/90 – Huntington Beach, CA

Huntington Beach Tars

and Spilled Oil

Other Southern California

Beach Tars

Alaska Crude Oils

California Crude Oils

Correlation Using Bulk Isotope Ratios

Contamination in monitoring wells had two possible sources; GC fingerprints were similar since both were contemporary gasolines; isotopically distinct since derived from different crude oils

d13C (‰)

dD (‰)

MW-2

MW-5

MW-11

MW-4

Brand A Gasoline

Brand B

Gasoline

RITS Fall 2008: Utilization of Stable Isotopes…

*

Brand A gasoline clearly different than brand B gasoline. Gasoline in groundwater degraded relative to Brand A and cannot be from Brand B. This is an example where the bulk C and H isotopes were determined on the gasoline samples and used to discriminate samples of different origins and to relate one source to the samples in the monitoring wells.

EXXON VALDEZ

  • March 24th, 1989
  • 258,000 barrels of Alaskan North Slope crude oil spilled into Prince William Sound

Residues from Prince William Sound

Terpanes in Prince William Sound Residues

-24.5

-29.1

-28.7

-24.1

*

Stable Isotope Determinations

ISOTOPIC VALUES CAN BE MEASURED IN TWO WAYS:

  • BULK ISOTOPES
  • ISOTOPIC COMPOSITIONS OF INDIVIDUAL COMPOUNDS

GCIRMS System

*

Crude Oil Chromatogram

0

C17

C35

Pristane

Phytane

*

GCIRMS DATA FOR SELECTED

OILS

n-alkanes

-35

-34

-33

-32

-31

-30

-29

-28

-27

-26

-25

C13

C15

C17

C19

C21

C23

C25

C27

C29

C31

C33

C35

24D

36D

d

13C (‰)

U8-106-1

Paris Basin

Middle East

Oklahoma

Mahakam

*

Hydrocarbon Spills and Weathering

  • Major effects of weathering from a geochemical perspective are :
  • Evaporation
  • Water washing
  • Biodegradation

*

Tar Ball Chromatograms

124.unknown

*

Terpanes in Tar Ball Samples

18a-Oleanane

C29-Hop.

C30-Hop.

*

GCIRMS – Tar Balls

*

Sampling locations of oil residues

and oiled bird feathers collected along

the Atlantic Coast of France after the Erika oil spill.

Mazeas et al., EST, 36(2), 130-137, 2002

The Erika Oil Spill.

*

Bulk isotope values

The Erika Oil Spill.

*

Molecular n-alkane isotopic compositions of the oil residues collected in the north Atlantic shoreline (mean of S2-S12), on the Crohot Beach (S13), in the Arcachon Bay area (mean of S14-S18), and of bird feathers (mean of S19-S28) are compared with Erika oil.

The Erika Oil Spill.

*

                                                                          

Compound specific isotopic composition of oil residues and oiled bird feathers collected along the Atlantic Coast of France compared with Erika oil isotopic composition.

The Erika Oil Spill.

*

Diesel Fingerprints

*

Isoprenoid Isotope Fingerprints

California

Oklahoma

Chart1

C14i C14i
C15i C15i
C16i C16i
C18i C18i
PR PR
PH PH
B
C
Carbon Isotope Value
-21.65
-25.54
-23.62
-26.57
-25.48
-30.22
-25.1
-29.57
-26.09
-31.39
-25.2
-29.84

Sheet1

ITT/MW16* ITT/MW16* RW-27* RW-27* RW-41* RW-41* TEX. DIESEL TEX. DIESEL JP-8 JP-8
9198 9200 9195A 9197 9195B 9199 9201 9203 9202 9204
C9D -31.58 -30.95 -31.44 -31.25 -31.39 -31.35 -31.89 -31.15 -31.43 -31.62
C10D -32.42 -32.43 -32.41 -32.36 -32.38 -32.45 -33.41 -32.46 -32.74 -32.79
C11
C12 -24.45 -24.5 -24.23 -23.46 -23.11 -22.78 -28.69 -27.53 -27.94 -27.13
C14i -21.28 -21.65 -21.54 -21.6 -21.26 -25.54 -24.74 -26.83 -25.67
C13 -25.27 -25.01 -24.23 -25.43 -23.84 -24.32 -31.13 -30.92 -29.43 -28.35
C15i -24.58 -24.6 -23.62 -24.07 -24.13 -25.21 -26.57 -26.75 -27.2 -27.26
C14 -24.54 -24.95 -26.02 -26.3 -24.73 -24.95 -28.33 -28.08 -28.69 -28.36
C16i -25.92 -25.91 -25.48 -25.5 -25.6 -25.87 -30.22 -29.48 -29.56 -29.49
C15 -25.02 -25.14 -25.61 -25.64 -25.45 -29.11 -28.81 -28.86 -28.82
C16D -30.11 -30.18 -30.09 -30.3 -30.28 -30.07 -31.02 -29.98 -30.64 -30.44
C16 -25.04 -24.76 -26.36 -24.81 -24.97 -28.03 -28.53 -28.34 -28.15
C18i -24.96 -25.26 -25.1 -25.01 -25.12 -24.72 -29.57 -30.83
C17 -24.95 -25.05 -25.96 -25.54 -24.99 -25.13 -33.41 -33.72
PR -25.02 -24.99 -26.09 -25.9 -25.61 -25.75 -31.39 -31.67
C18 -25.04 -25.19 -25.54 -25.37 -25.22 -25.18 -28.34 -28.04
PH -24.2 -24.09 -25.2 -24.72 -25.04 -25.03 -29.84 -30.96
C19D -26.34 -26.45 -26.51 -26.49 -27.11 -26.68 -28.27 -28.31 -27.42 -27.26
C19 -29.06 -27.64 -24.89 -25.45 -24.82 -30 -30.55
C20 -25.71 -25.62 -25.39 -27.97 -28.51
C21 -28.49 -28.23
C22 -27.91 -28.78
C24D -23.18 -24.73 -26.07 -24.67 -25.61 -25.39 -26.44 -24.19 -26.26 -29.4

Sheet2

ITT/MW16* ITT/MW16* RW-27* RW-27* RW-41* RW-41* TEX. DIESEL TEX. DIESEL JP-8 JP-8 B C
C14i -21.28 -21.65 -21.54 -21.6 -21.26 -25.54 -24.74 -26.83 -25.67 C14i -21.65 -25.54
C15i -24.58 -24.6 -23.62 -24.07 -24.13 -25.21 -26.57 -26.75 -27.2 -27.26 C15i -23.62 -26.57
C16i -25.92 -25.91 -25.48 -25.5 -25.6 -25.87 -30.22 -29.48 -29.56 -29.49 C16i -25.48 -30.22
C18i -24.96 -25.26 -25.1 -25.01 -25.12 -24.72 -29.57 -30.83 C18i -25.1 -29.57
PR -25.02 -24.99 -26.09 -25.9 -25.61 -25.75 -31.39 -31.67 PR -26.09 -31.39
PH -24.2 -24.09 -25.2 -24.72 -25.04 -25.03 -29.84 -30.96 PH -25.2 -29.84
C9D -31.58 -30.95 -31.44 -31.25 -31.39 -31.35 -31.89 -31.15 -31.43 -31.62 C9D -31.44 -31.89
C10D -32.42 -32.43 -32.41 -32.36 -32.38 -32.45 -33.41 -32.46 -32.74 -32.79 C10D -32.41 -33.41
C16D -30.11 -30.18 -30.09 -30.3 -30.28 -30.07 -31.02 -29.98 -30.64 -30.44 C16D -30.09 -31.02
C19D -26.34 -26.45 -26.51 -26.49 -27.11 -26.68 -28.27 -28.31 -27.42 -27.26 C19D -26.51 -28.27
C24D -23.18 -24.73 -26.07 -24.67 -25.61 -25.39 -26.44 -24.19 -26.26 -29.4 C24D -26.07 -26.44

Sheet2

0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
ITT/MW16*
ITT/MW16*
RW-27*
RW-27*
RW-41*
RW-41*
TEX. DIESEL
TEX. DIESEL
JP-8
JP-8

Sheet3

0 0 0
0 0 0
0 0 0
0 0 0
0 0 0
0 0 0
B
C
Compound
Carbon Isotope Value
Diesel Isoprenoids
ITT/MW16* ITT/MW16* RW-27* RW-27* RW-41* RW-41* TEX. DIESEL TEX. DIESEL JP-8 JP-8
C12 -24.45 -24.5 -24.23 -23.46 -23.11 -22.78 -28.69 -27.53 -27.94 -27.13
C13 -25.27 -25.01 -24.23 -25.43 -23.84 -24.32 -31.13 -30.92 -29.43 -28.35
C14 -24.54 -24.95 -26.02 -26.3 -24.73 -24.95 -28.33 -28.08 -28.69 -28.36
C15 -25.02 -25.14 -25.61 -25.64 -25.45 -29.11 -28.81 -28.86 -28.82
C16 -25.04 -24.76 -26.36 -24.81 -24.97 -28.03 -28.53 -28.34 -28.15
C17 -24.95 -25.05 -25.96 -25.54 -24.99 -25.13 -33.41 -33.72
C18 -25.04 -25.19 -25.54 -25.37 -25.22 -25.18 -28.34 -28.04
C19 -29.06 -27.64 -24.89 -25.45 -24.82 -30 -30.55
C20 -25.71 -25.62 -25.39 -27.97 -28.51
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
ITT/MW16*
ITT/MW16*
RW-27*
RW-27*
RW-41*
RW-41*
TEX. DIESEL
TEX. DIESEL
JP-8
JP-8

*

Forensic Geochemistry

Site A

Site B

Groundwater flow direction

MW 1

B

C

MW6

*

Weathered and Unweathered Diesel

Diesel MW 6

Diesel MW 1

Pr

Ph

C17

*

*

*

*

*

*

*

Carbon Isotope Values for Isoprenoids

Chart1

C14i C14i
C15i C15i
C16i C16i
C18i C18i
PR PR
PH PH
MW 6
MW 1
-21.28
-21.65
-24.28
-23.62
-25.92
-25.48
-24.96
-25.1
-25.32
-25.89
-24.2
-25.2

Sheet1

ITT/MW16* ITT/MW16* RW-27* RW-27* RW-41* RW-41* TEX. DIESEL TEX. DIESEL JP-8 JP-8
9198 9200 9195A 9197 9195B 9199 9201 9203 9202 9204
C9D -31.58 -30.95 -31.44 -31.25 -31.39 -31.35 -31.89 -31.15 -31.43 -31.62
C10D -32.42 -32.43 -32.41 -32.36 -32.38 -32.45 -33.41 -32.46 -32.74 -32.79
C11
C12 -24.45 -24.5 -24.23 -23.46 -23.11 -22.78 -28.69 -27.53 -27.94 -27.13
C14i -21.28 -21.65 -21.54 -21.6 -21.26 -25.54 -24.74 -26.83 -25.67
C13 -25.27 -25.01 -24.23 -25.43 -23.84 -24.32 -31.13 -30.92 -29.43 -28.35
C15i -24.58 -24.6 -23.62 -24.07 -24.13 -25.21 -26.57 -26.75 -27.2 -27.26
C14 -24.54 -24.95 -26.02 -26.3 -24.73 -24.95 -28.33 -28.08 -28.69 -28.36
C16i -25.92 -25.91 -25.48 -25.5 -25.6 -25.87 -30.22 -29.48 -29.56 -29.49
C15 -25.02 -25.14 -25.61 -25.64 -25.45 -29.11 -28.81 -28.86 -28.82
C16D -30.11 -30.18 -30.09 -30.3 -30.28 -30.07 -31.02 -29.98 -30.64 -30.44
C16 -25.04 -24.76 -26.36 -24.81 -24.97 -28.03 -28.53 -28.34 -28.15
C18i -24.96 -25.26 -25.1 -25.01 -25.12 -24.72 -29.57 -30.83
C17 -24.95 -25.05 -25.96 -25.54 -24.99 -25.13 -33.41 -33.72
PR -25.02 -24.99 -26.09 -25.9 -25.61 -25.75 -31.39 -31.67
C18 -25.04 -25.19 -25.54 -25.37 -25.22 -25.18 -28.34 -28.04
PH -24.2 -24.09 -25.2 -24.72 -25.04 -25.03 -29.84 -30.96
C19D -26.34 -26.45 -26.51 -26.49 -27.11 -26.68 -28.27 -28.31 -27.42 -27.26
C19 -29.06 -27.64 -24.89 -25.45 -24.82 -30 -30.55
C20 -25.71 -25.62 -25.39 -27.97 -28.51
C21 -28.49 -28.23
C22 -27.91 -28.78
C24D -23.18 -24.73 -26.07 -24.67 -25.61 -25.39 -26.44 -24.19 -26.26 -29.4

Sheet2

ITT/MW16* ITT/MW16* RW-27* RW-27* RW-41* RW-41* TEX. DIESEL TEX. DIESEL JP-8 JP-8
C14i -21.28 -21.65 -21.54 -21.6 -21.26 -25.54 -24.74 -26.83 -25.67
C15i -24.58 -24.6 -23.62 -24.07 -24.13 -25.21 -26.57 -26.75 -27.2 -27.26
C16i -25.92 -25.91 -25.48 -25.5 -25.6 -25.87 -30.22 -29.48 -29.56 -29.49
C18i -24.96 -25.26 -25.1 -25.01 -25.12 -24.72 -29.57 -30.83
PR -25.02 -24.99 -26.09 -25.9 -25.61 -25.75 -31.39 -31.67
PH -24.2 -24.09 -25.2 -24.72 -25.04 -25.03 -29.84 -30.96
C9D -31.58 -30.95 -31.44 -31.25 -31.39 -31.35 -31.89 -31.15 -31.43 -31.62
C10D -32.42 -32.43 -32.41 -32.36 -32.38 -32.45 -33.41 -32.46 -32.74 -32.79
C16D -30.11 -30.18 -30.09 -30.3 -30.28 -30.07 -31.02 -29.98 -30.64 -30.44
C19D -26.34 -26.45 -26.51 -26.49 -27.11 -26.68 -28.27 -28.31 -27.42 -27.26
C24D -23.18 -24.73 -26.07 -24.67 -25.61 -25.39 -26.44 -24.19 -26.26 -29.4
MW 6 MW 1
C14i -21.28 -21.65
C15i -24.28 -23.62
C16i -25.92 -25.48
C18i -24.96 -25.1
PR -25.32 -25.89
PH -24.2 -25.2

Sheet2

0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
ITT/MW16*
ITT/MW16*
RW-27*
RW-27*
RW-41*
RW-41*
TEX. DIESEL
TEX. DIESEL
JP-8
JP-8

Sheet3

0 0
0 0
0 0
0 0
0 0
0 0
MW 6
MW 1
ITT/MW16* ITT/MW16* RW-27* RW-27* RW-41* RW-41* TEX. DIESEL TEX. DIESEL JP-8 JP-8
C12 -24.45 -24.5 -24.23 -23.46 -23.11 -22.78 -28.69 -27.53 -27.94 -27.13
C13 -25.27 -25.01 -24.23 -25.43 -23.84 -24.32 -31.13 -30.92 -29.43 -28.35
C14 -24.54 -24.95 -26.02 -26.3 -24.73 -24.95 -28.33 -28.08 -28.69 -28.36
C15 -25.02 -25.14 -25.61 -25.64 -25.45 -29.11 -28.81 -28.86 -28.82
C16 -25.04 -24.76 -26.36 -24.81 -24.97 -28.03 -28.53 -28.34 -28.15
C17 -24.95 -25.05 -25.96 -25.54 -24.99 -25.13 -33.41 -33.72
C18 -25.04 -25.19 -25.54 -25.37 -25.22 -25.18 -28.34 -28.04
C19 -29.06 -27.64 -24.89 -25.45 -24.82 -30 -30.55
C20 -25.71 -25.62 -25.39 -27.97 -28.51
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0
ITT/MW16*
ITT/MW16*
RW-27*
RW-27*
RW-41*
RW-41*
TEX. DIESEL
TEX. DIESEL
JP-8
JP-8

Gasolines

  • Gasolines from different sources often have very similar chromatograms, making it difficult to distinguish such samples
  • Gasolines are also devoid of biomarkers, further limiting correlation possibilities
  • One solution here is to use GCIRMS for both the hydrocarbons and additives

RITS Fall 2008: Utilization of Stable Isotopes…

*

This is another example of the advantage of using isotope compositions of individual compounds in gasolines. Again gasolines are chromatographically very similar even when coming from different sources. Hence GC and GCMS are of relatively little use in discriminating samples from different sources. However if the gasolines are derived from different crude oils it may be possible that the isotopic fingerprints can be used to differentiate the products.

*

Comparison of Gasolines by GC

*

30

31

52

62

66

67

78

83

89

90

91

94

97

102

107

109

113

108

114

117

118

119

122

123

126

133

135

136

138

143

144

148

149

150

151

152

Gasoline Database

Retail stations locations

Aromatics δ13C + dD, oxygenates (MTBE, TBA)

Aromatics δ13C only

138

108

Samples provided by Dr. J.Graham Rankin,

Marshall University, WV

*

δ13C Fingerprints of 39 Gasolines

-29

-28

-27

-26

-25

-24

-23

-22

1,3,5-TMB

m/p-X

TOL

1,2,4-TMB

o-X

EB

1m2eBz

1m3eBz

1,2,3-TMB

proBz

Naph

A

H

δ13C (‰)

Trend reflects the manufacturing process

δ13C offset reflects the crude oil feedstock

Crude oil feedstock variable

CSIA of Gasoline

ethylbenzene

δ13C = –24.6 ‰

m,p-xylene

δ13C = –26.0 ‰

o-xylene

δ13C = –25.1 ‰

1,2,4-trimethylbenzene

δ13C = –26.7 ‰

RITS Fall 2008: Utilization of Stable Isotopes…

*

In a previous slide the bulk isotope values for a brand A and brand B gasoline were given. However these bulk values are the weighted averages of the isotopic compositions of all the individual compounds in the gasolines. Here is a portion of a gasoline chromatogram that has been analysed by GCIRMS and the isotopic compositions of several individual compounds are shown. The variations in these individual compounds highlights the fact that the isotopic fingerprint potentially provides another tool that can be used to differentiate products from different sources providing far more specificity than the bulk values alone.

Gasolines – GC Signals

mp-Xyl

1,2,4-TMB

A

B

RITS Fall 2008: Utilization of Stable Isotopes…

*

Partial chromatograms showing the similarity in these two gasoline products.

*

Gasolines – Different δ13C Fingerprints

-30

-29

-28

-27

-26

-25

-24

-23

-22

1,3,5-TMB

m/p-X

TOL

1,2,4-TMB

o-X

EB

1m2eBz

1m3eBz

1,2,3-TMB

proBz

Naph

A

H

A

B

δ13C (‰)

PCE Degradation Site Study

Hunkeler et al., J. Contaminant Hydrology, 74, 265-282,2004.

Hunkeler et al., J. Contaminant Hydrology, 74, 265-282,2004.

PCE Source Evaluation Study

-33

-27

-25

The cross section close to the source shows that now instead of one homogeneous source there are three isotopically distinct sources across the transect. These three may represent multiple sources or multiple releases from the same source. These variations did not result from biodegradation since no degradation products were observed.

*

PCBs

  • Study by Yanik et al., OG, 239-253, 34, 2003 showed that different Aroclors may be isotopically different and thus useful for source discrimination although there is some slight enrichment from degradation.

*

M/z 44 Chromatogram for Aroclor 1245

*

Variations in Isotopic Composition of Various Congeners

PAHs and Stable Isotopes

  • Current interest is centered around whether carbon isotopes can be used to discriminate PAHs derived from former manufactured gas plant (MGP) wastes versus those from general urban background aromatics
  • Urban backgrounds have a fairly narrow range and small differences may be related to source differences

*

*

Sources of PAHs to Urban Background

Mixed Pyrogenic and Petrogenic Sources

*

PAHs-Combined GC and GCIRMS Data

07

08

Chart2

14 14
15 15
16 16
17 17
18 18
19 19
20 20
21 21
22 22
23 23
24 24
25 25
26 26
27,28 27,28
29 29
30,31 30,31
32 32
9D 9D
10D 10D
16D 16D
19D 19D
24D 24D
32D 32D
7
8
Peak ID
Carbon isotope value
-25.44
-25.598
-24.763
-25.48
-24.906
-25.96
-22.33
-21.55
-28.81
-24.5905
-24.405
-23.275
-21.87
-25.52
-25.301
-24.9955
-24.995
-25.6365
-26.159
-25.1585
-23.565
-24.64
-24.4845
-24.8655
-25.725
-24.73
-24.75
-31.632
-31.6535
-32.868
-33.0925
-29.03
-30.951
-27.175
-27.783
-26.81
-27.187
-30.315
-30.817

PAH DATA

1034 1047 1057 1066 1082 AVERAGE STDEVP 1038 1065 AVERAGE STDEVP 1052 1064 1080 AVERAGE STDEVP 1037 1063 AVERAGE STDEVP 1051 1062 AVERAGE STDEVP 1032 1079 AVERAGE STDEVP 1033 1060 AVERAGE STDEVP 1036 1059 AVERAGE STDEVP 1050 1058 AVERAGE STDEVP 1031 1055 AVERAGE STDEVP 1049 1056 AVERAGE STDEVP 1039 1077 AVERAGE STDEVP 1048 1076 AVERAGE STDEVP 1053 1075 AVERAGE STDEVP 1045 1073 AVERAGE STDEVP 1044 1072 AVERAGE STDEVP 1043 1071 1081 AVERAGE STDEVP 1042 1070 AVERAGE STDEVP 1054 1069 AVERAGE STDEVP 1041 1068 AVERAGE STDEVP 1040 1067 1078 AVERAGE STDEVP
OU4007 OU4007 OU4007 OU4007 OU4007 OU4007 OU4007 GTO31010-01 GTO31010-01 GTO31010-01 GTO31010-01 GTO31010-02 GTO31010-02 GTO31010-02 GTO31010-02 GTO31010-02 GTO31010-03 GTO31010-03 GTO31010-03 GTO31010-03 GTO31010-04 GTO31010-04 GTO31010-04 GTO31010-04 GTO31010-05 GTO31010-05 GTO31010-05 GTO31010-05 GTO31010-06 GTO31010-06 GTO31010-06 GTO31010-06 GTO31010-07 GTO31010-07 GTO31010-07 GTO31010-07 GTO31010-08 GTO31010-08 GTO31010-08 GTO31010-08 GTO31010-09 GTO31010-09 GTO31010-09 GTO31010-09 GTO31010-10 GTO31010-10 GTO31010-10 GTO31010-10 GTO31010-11 GTO31010-11 GTO31010-11 GTO31010-11 GTO31010-12 GTO31010-12 GTO31010-12 GTO31010-12 GTO31010-13 GTO31010-13 GTO31010-13 GTO31010-13 GTO31010-14 GTO31010-14 GTO31010-14 GTO31010-14 GTO31010-15 GTO31010-15 GTO31010-15 GTO31010-15 GTO31010-16 GTO31010-16 GTO31010-16 GTO31010-16 GTO31010-16 GTO31010-17 GTO31010-17 GTO31010-17 GTO31010-17 GTO31010-18 GTO31010-18 GTO31010-18 GTO31010-18 GTO31010-19 GTO31010-19 GTO31010-19 GTO31010-19 GTO31010-20 GTO31010-20 GTO31010-20 GTO31010-20 GTO31010-20
3 622 -30.84 -29.46 -29.82 -28.87 -30.14 -29.83 0.66 9D 683 -31.31 -31.12 -31.22 0.10 9D 683 -31.43 -31.63 -31.18 -31.41 0.19 9D 683 -31.60 -31.59 -31.59 0.01 9D 683 -31.48 -31.61 -31.54 0.06 9D 683 -31.19 -31.61 -31.40 0.21 9D 683 -31.46 -31.43 -31.45 0.02 9D 683 -31.81 -31.46 -31.63 0.18 9D 683 -31.53 -31.78 -31.65 0.12 9D 683 -31.40 -31.53 -31.46 0.06 9D 683 -31.49 -31.61 -31.55 0.06 9D 683 -30.56 -30.84 -30.70 0.14 9D 683 -31.73 -31.81 -31.77 0.04 9D 683 -31.60 -31.44 -31.52 0.08 9D 683 -31.52 -31.33 -31.42 0.10 9D 683 -31.62 -31.55 -31.58 0.04 9D 683 -30.78 -31.04 -30.91 -30.91 0.11 9D 683 -31.62 -31.54 -31.58 0.04 9D 683 -31.49 -31.57 -31.53 0.04 9D 683 -31.55 -32.37 -31.96 0.41 9D 683 -30.92 -31.01 -30.97 0.04
4 642 -26.94 -26.74 -26.52 -26.60 -26.80 -26.72 0.15 10D 932 -32.97 -33.34 -33.16 0.18 10D 932 -33.13 -33.03 -32.78 -32.98 0.14 10D 932 -33.04 -33.27 -33.15 0.12 10D 932 -33.04 -32.95 -32.99 0.05 10D 932 -32.74 -33.10 -32.92 0.18 10D 932 -32.93 -32.83 -32.88 0.05 10D 932 -33.20 -32.54 -32.87 0.33 10D 932 -33.01 -33.17 -33.09 0.08 10D 932 -33.44 -33.05 -33.25 0.19 10D 932 -33.29 -33.10 -33.20 0.10 10D 932 -33.04 -32.95 -33.00 0.04 10D 932 -32.85 -33.07 -32.96 0.11 10D 932 -32.46 -32.75 -32.60 0.14 10D 932 -33.07 -32.85 -32.96 0.11 10D 932 -33.01 -33.06 -33.03 0.03 10D 932 -32.72 -32.93 -32.81 -32.82 0.08 10D 932 -32.95 -33.01 -32.98 0.03 10D 932 -33.19 -32.98 -33.09 0.10 10D 932 -32.94 -32.34 -32.64 0.30 10D 932 -32.82 -32.71 -33.05 -32.86 0.14
9D 683 -29.40 -29.57 -30.27 -30.07 -29.66 -29.79 0.32 14 1409 14 1409 14 1409 14 1409 14 1409 -25.61 -25.45 -25.53 0.08 14 1409 -25.06 -25.26 -25.16 0.10 14 1409 -25.48 -25.40 -25.44 0.04 14 1409 -25.58 -25.62 -25.60 0.02 14 1409 14 1409 14 1409 14 1409 14 1409 14 1409 14 1409 14 1409 -25.80 -25.09 -25.17 -25.35 0.32 14 1409 -25.38 -25.23 -25.31 0.08 14 1409 14 1409 -24.60 -25.40 -25.00 0.40 14 1409 -24.61 -24.61 0.00
5,6 703 -26.57 -27.10 -27.17 -26.99 -27.27 -27.02 0.24 15 1644 15 1644 15 1644 15 1644 15 1644 -24.78 -26.75 -25.76 0.99 15 1644 -24.54 -24.54 0.00 15 1644 -24.62 -24.91 -24.76 0.15 15 1644 -25.48 -25.48 0.00 15 1644 15 1644 15 1644 15 1644 15 1644 15 1644 15 1644 15 1644 -24.80 -24.57 -25.12 -24.83 0.22 15 1644 -24.62 -24.54 -24.58 0.04 15 1644 15 1644 15 1644 -24.37 -24.49 -24.48 -24.45 0.05
7 784 -27.58 -27.11 -27.28 -27.17 -27.19 -27.27 0.17 16 1680 16 1680 16 1680 16 1680 16 1680 -25.17 -26.24 -25.71 0.53 16 1680 -26.70 -26.70 0.00 16 1680 -25.08 -24.74 -24.91 0.17 16 1680 -26.15 -25.77 -25.96 0.19 16 1680 16 1680 16 1680 16 1680 16 1680 16 1680 16 1680 16 1680 -24.53 -24.24 -25.25 -24.67 0.42 16 1680 -24.46 -24.71 -24.59 0.13 16 1680 16 1680 16 1680 -24.73 -25.14 -25.80 -25.22 0.44
8 858 -27.33 -28.15 -28.31 -28.15 -28.15 -28.02 0.35 17 1950 17 1950 17 1950 -26.08 -26.08 0.00 17 1950 17 1950 -24.33 -24.64 -24.49 0.15 17 1950 -24.65 -25.49 -25.07 0.42 17 1950 -22.63 -22.03 -22.33 0.30 17 1950 -21.55 -21.55 0.00 17 1950 17 1950 17 1950 17 1950 17 1950 17 1950 17 1950 17 1950 -21.42 -20.90 -21.32 -21.21 0.23 17 1950 -21.41 -21.05 -21.23 0.18 17 1950 17 1950 17 1950 -23.12 -22.58 -22.67 -22.79 0.24
9 894 -28.56 -27.65 -27.94 -27.98 -27.71 -27.97 0.32 18 2016 18 2016 18 2016 18 2016 18 2016 -29.82 -29.82 0.00 18 2016 18 2016 -29.30 -28.32 -28.81 0.49 18 2016 18 2016 18 2016 18 2016 18 2016 18 2016 18 2016 18 2016 18 2016 -25.14 -25.46 -25.30 0.16 18 2016 -28.98 -29.39 -29.19 0.20 18 2016 18 2016 18 2016 -26.05 -28.28 -27.17 1.12
10D 932 -31.95 -32.55 -32.66 -32.21 -32.45 -32.36 0.25 19 2071 19 2071 19 2071 19 2071 19 2071 -24.32 -23.87 -24.10 0.23 19 2071 -24.09 -23.96 -24.02 0.07 19 2071 -24.62 -24.56 -24.59 0.03 19 2071 -24.67 -24.14 -24.41 0.26 19 2071 19 2071 19 2071 19 2071 19 2071 -24.41 -24.41 0.00 19 2071 19 2071 19 2071 -24.67 -24.41 -24.42 -24.50 0.12 19 2071 -24.07 -23.96 -24.02 0.05 19 2071 19 2071 -23.57 -23.40 -23.49 0.08 19 2071 -23.73 -24.92 -23.90 -24.18 0.53
10 957 -27.54 -27.40 -27.25 -27.02 -27.16 -27.27 0.18 16D 2151 -30.96 -31.22 -31.09 0.13 16D 2151 -31.13 -31.13 -31.04 -31.10 0.04 16D 2151 -29.89 -30.11 -30.00 0.11 16D 2151 -31.14 -31.16 -31.15 0.01 16D 2151 -29.36 -29.35 -29.36 0.00 16D 2151 -30.22 -29.99 -30.11 0.11 16D 2151 -29.00 -29.06 -29.03 0.03 16D 2151 -30.92 -30.98 -30.95 0.03 16D 2151 -30.94 -30.76 -30.85 0.09 16D 2151 -31.04 -31.12 -31.08 0.04 16D 2151 -31.08 -31.00 -31.04 0.04 16D 2151 -30.30 -30.78 -30.54 0.24 16D 2151 -30.29 -30.30 -30.30 0.00 16D 2151 -31.23 -31.06 -31.14 0.08 16D 2151 -30.68 -30.50 -30.59 0.09 16D 2151 -29.21 -29.50 -29.35 -29.35 0.12 16D 2151 -29.75 -29.92 -29.84 0.08 16D 2151 -30.81 -30.74 -30.77 0.04 16D 2151 -30.81 -31.15 -30.98 0.17 16D 2151 -29.57 -29.73 -29.43 -29.58 0.12
11 1000 -23.63 -23.40 -23.50 -23.53 -23.34 -23.48 0.10 20 2191 20 2191 20 2191 -23.03 -23.14 -23.09 0.05 20 2191 20 2191 -22.28 -21.50 -21.89 0.39 20 2191 -21.28 -21.14 -21.21 0.07 20 2191 -23.55 -23.00 -23.28 0.28 20 2191 -21.80 -21.94 -21.87 0.07 20 2191 20 2191 20 2191 20 2191 20 2191 -22.05 -19.97 -21.01 1.04 20 2191 20 2191 20 2191 -23.50 -22.67 -24.65 -23.61 0.81 20 2191 -23.06 -24.00 -23.53 0.47 20 2191 -19.47 -29.15 -24.31 4.84 20 2191 -23.15 -23.15 0.00 20 2191 -24.77 -25.34 -24.82 -24.98 0.26
12 1033 -33.30 -32.71 -32.60 -32.37 -32.60 -32.72 0.31 21 2519 -25.02 -25.14 -25.08 0.06 21 2519 -25.97 -25.82 -25.90 0.08 21 2519 -25.56 -25.50 -25.53 0.03 21 2519 21 2519 -25.55 -25.30 -25.43 0.12 21 2519 -25.09 -25.01 -25.05 0.04 21 2519 -25.52 -25.53 -25.52 0.00 21 2519 -25.25 -25.35 -25.30 0.05 21 2519 -24.63 -24.38 -24.51 0.12 21 2519 21 2519 -24.86 -24.56 -24.71 0.15 21 2519 -24.76 -24.53 -24.64 0.11 21 2519 -24.82 -24.54 -24.68 0.14 21 2519 -25.39 -25.46 -25.43 0.03 21 2519 -26.08 -25.42 -25.75 0.33 21 2519 -25.72 -25.31 -24.91 -25.31 0.33 21 2519 -25.73 -25.50 -25.62 0.11 21 2519 21 2519 -24.84 -24.87 -24.86 0.02 21 2519 -24.58 -25.53 -24.84 -24.98 0.40
13 1109 -26.58 -25.84 -26.10 -26.36 -26.06 -26.19 0.26 22 2535 22 2535 22 2535 -25.18 -25.15 -25.16 0.02 22 2535 22 2535 -24.62 -24.98 -24.80 0.18 22 2535 -24.41 -24.29 -24.35 0.06 22 2535 -24.99 -25.00 -25.00 0.00 22 2535 -25.01 -24.98 -25.00 0.02 22 2535 -25.00 -25.14 -25.07 0.07 22 2535 22 2535 -24.58 -24.58 0.00 22 2535 -24.60 -25.31 -24.96 0.36 22 2535 -24.61 -24.48 -24.55 0.07 22 2535 -23.85 -23.85 0.00 22 2535 -24.08 -24.83 -24.46 0.37 22 2535 -24.20 -24.46 -24.79 -24.48 0.24 22 2535 -24.80 -24.87 -24.84 0.04 22 2535 22 2535 -24.47 -24.11 -24.29 0.18 22 2535 -24.07 -24.70 -24.73 -24.50 0.31
14 1409 -25.55 -24.98 -25.20 -25.22 -25.02 -25.20 0.20 19D 2625 -27.79 -27.98 -27.88 0.10 19D 2625 -28.04 -27.86 -27.93 -27.94 0.08 19D 2625 -27.15 -27.34 -27.25 0.10 19D 2625 -28.04 -27.96 -28.00 0.04 19D 2625 -28.04 -28.43 -28.23 0.19 19D 2625 -27.66 -27.85 -27.76 0.10 19D 2625 -27.19 -27.16 -27.18 0.02 19D 2625 -27.80 -27.77 -27.78 0.01 19D 2625 -27.48 -27.80 -27.64 0.16 19D 2625 -28.30 -27.98 -28.14 0.16 19D 2625 -27.67 -27.41 -27.54 0.13 19D 2625 -27.62 -27.54 -27.58 0.04 19D 2625 -27.35 -27.30 -27.33 0.02 19D 2625 -27.58 -27.06 -27.32 0.26 19D 2625 -27.22 -27.97 -27.60 0.38 19D 2625 -27.51 -27.21 -27.23 -27.32 0.14 19D 2625 -27.57 -27.50 -27.54 0.03 19D 2625 -28.11 -27.68 -27.89 0.22 19D 2625 -27.59 -27.83 -27.71 0.12 19D 2625 -28.26 -27.77 -27.76 -27.93 0.23
15 1644 -24.00 -23.83 -23.84 -23.36 -24.08 -23.82 0.25 23 2938 -25.54 -25.50 -25.52 0.02 23 2938 -28.26 -27.87 -28.07 0.19 23 2938 -26.18 -26.08 -26.13 0.05 23 2938 -30.07 -29.55 -29.81 0.26 23 2938 -25.82 -25.13 -25.48 0.34 23 2938 -25.71 -25.65 -25.68 0.03 23 2938 -25.88 -25.39 -25.64 0.24 23 2938 -26.32 -26.00 -26.16 0.16 23 2938 -25.18 -24.85 -25.02 0.17 23 2938 23 2938 -25.02 -25.45 -25.24 0.22 23 2938 -25.00 -25.17 -25.08 0.08 23 2938 -25.37 -25.21 -25.29 0.08 23 2938 -25.41 -25.27 -25.34 0.07 23 2938 -25.39 -25.49 -25.44 0.05 23 2938 -26.26 -25.95 -25.63 -25.95 0.26 23 2938 -25.76 -25.66 -25.71 0.05 23 2938 23 2938 -25.26 -25.23 -25.24 0.02 23 2938 -24.86 -25.11 -24.70 -24.89 0.17
FS1 1659 -23.65 -23.46 -23.05 -24.10 -22.00 -23.25 0.71 24 3008 -22.51 -22.58 -22.55 0.03 24 3008 -29.38 -29.23 -29.31 0.08 24 3008 -25.87 -26.03 -25.95 0.08 24 3008 24 3008 -25.66 -25.04 -25.35 0.31 24 3008 -25.35 -24.88 -25.12 0.23 24 3008 -25.40 -24.92 -25.16 0.24 24 3008 -23.75 -23.38 -23.57 0.19 24 3008 -24.95 -24.61 -24.78 0.17 24 3008 24 3008 -24.69 -24.91 -24.80 0.11 24 3008 -24.89 -25.14 -25.01 0.13 24 3008 24 3008 -24.99 -24.90 -24.94 0.05 24 3008 -25.77 -25.31 -25.54 0.23 24 3008 -22.00 -21.42 -22.35 -21.92 0.38 24 3008 -22.65 -22.05 -22.35 0.30 24 3008 24 3008 -24.87 -25.22 -25.04 0.18 24 3008 -24.51 -23.46 -24.04 -24.00 0.43
16 1680 -30.60 -31.70 -31.49 -30.98 -31.87 -31.33 0.47 24D 3284 -26.84 -26.86 -26.85 0.01 24D 3284 -27.19 -27.05 -27.12 0.07 24D 3284 -26.55 -26.22 -26.39 0.17 24D 3284 -27.28 -27.11 -27.20 0.09 24D 3284 -26.98 -27.01 -27.00 0.02 24D 3284 -27.94 -26.83 -27.39 0.56 24D 3284 -26.88 -26.74 -26.81 0.07 24D 3284 -27.17 -27.2 -27.19 0.01 24D 3284 -27.08 -26.86 -26.97 0.11 24D 3284 -27.80 -27.35 -27.58 0.23 24D 3284 -26.70 -26.42 -26.56 0.14 24D 3284 -26.88 -26.83 -26.86 0.03 24D 3284 -25.24 -24.90 -25.07 0.17 24D 3284 -26.81 -26.67 -26.74 0.07 24D 3284 -26.65 -26.73 -26.69 0.04 24D 3284 -26.47 -26.67 -26.34 -26.49 0.14 24D 3284 -26.42 -26.60 -26.51 0.09 24D 3284 -27.08 -27.25 -27.16 0.08 24D 3284 -26.74 -27.54 -27.14 0.40 24D 3284 -26.24 -26.50 -26.50 -26.41 0.12
SS2 1785 -31.39 -31.53 -31.65 -31.53 -31.45 -31.51 0.09 25 3432 -24.65 -24.27 -24.46 0.19 25 3432 -25.25 -25.25 0.00 25 3432 -24.74 -24.59 -24.67 0.08 25 3432 25 3432 -24.06 -23.55 -23.80 0.25 25 3432 -24.48 -23.99 -24.23 0.25 25 3432 -24.62 -24.66 -24.64 0.02 25 3432 25 3432 -23.94 -23.91 -23.93 0.01 25 3432 25 3432 -23.90 -24.04 -23.97 0.07 25 3432 -24.05 -24.24 -24.15 0.09 25 3432 -24.31 -23.86 -24.09 0.22 25 3432 -24.61 -24.71 -24.66 0.05 25 3432 -24.86 -24.58 -24.72 0.14 25 3432 -24.55 -24.38 -24.27 -24.40 0.12 25 3432 -24.38 -24.45 -24.42 0.03 25 3432 25 3432 -25.74 -24.22 -24.98 0.76 25 3432 -23.80 -23.93 -23.87 0.07
17 1950 -22.54 -23.38 -23.11 -22.15 -23.41 -22.92 0.49 26 3446 -24.73 -24.97 -24.85 0.12 26 3446 -25.27 -25.27 0.00 26 3446 -25.50 -25.44 -25.47 0.03 26 3446 26 3446 -24.59 -24.48 -24.53 0.05 26 3446 -24.66 -24.65 -24.65 0.01 26 3446 -24.49 -24.48 -24.48 0.01 26 3446 26 3446 -24.74 -24.39 -24.57 0.18 26 3446 26 3446 -24.90 -24.72 -24.81 0.09 26 3446 -24.90 -24.72 -24.81 0.09 26 3446 -24.90 -25.06 -24.98 0.08 26 3446 -24.68 -24.83 -24.75 0.08 26 3446 -24.73 -25.27 -25.00 0.27 26 3446 -25.15 -25.28 -25.22 -25.22 0.05 26 3446 -25.21 -25.45 -25.33 0.12 26 3446 26 3446 -24.37 -24.41 -24.39 0.02 26 3446 -24.73 -24.77 -24.75 0.02
FS2 1995 -25.87 -26.02 -26.03 -26.27 -26.00 -26.04 0.13 27,28 3787 -24.27 -24.91 -24.59 0.32 27,28 3787 27,28 3787 -25.62 -25.75 -25.69 0.06 27,28 3787 27,28 3787 -24.58 -24.51 -24.55 0.03 27,28 3787 -24.48 -24.51 -24.50 0.02 27,28 3787 -24.92 -24.81 -24.87 0.05 27,28 3787 27,28 3787 -24.69 -24.88 -24.79 0.09 27,28 3787 27,28 3787 -24.51 -24.82 -24.67 0.15 27,28 3787 -24.92 -25.18 -25.05 0.13 27,28 3787 -24.94 -24.77 -24.86 0.08 27,28 3787 -24.79 -24.94 -24.87 0.07 27,28 3787 -25.03 -25.01 -25.02 0.01 27,28 3787 -25.00 -25.12 -25.39 -25.17 0.16 27,28 3787 -25.30 -25.40 -25.35 0.05 27,28 3787 27,28 3787 -25.67 -25.22 -25.45 0.23 27,28 3787 -24.76 -24.60 -24.68 0.08
18 2016 -24.18 -24.86 -24.82 -24.02 -24.78 -24.53 0.36 29 3882 -25.76 -26.01 -25.88 0.13 29 3882 -26.74 -25.39 -24.74 -25.62 0.83 29 3882 -26.39 -26.26 -26.33 0.06 29 3882 29 3882 -24.93 -24.62 -24.77 0.16 29 3882 -24.93 -25.03 -24.98 0.05 29 3882 -25.87 -25.58 -25.73 0.14 29 3882 29 3882 -25.64 -25.95 -25.80 0.15 29 3882 29 3882 -25.84 -25.72 -25.78 0.06 29 3882 -25.52 -26.10 -25.81 0.29 29 3882 -26.26 -26.56 -26.41 0.15 29 3882 -25.54 -25.31 -25.43 0.12 29 3882 -24.91 -25.44 -25.17 0.27 29 3882 -25.21 -25.39 -25.30 0.09 29 3882 -25.25 -25.57 -25.41 0.16 29 3882 -24.65 -25.20 -24.93 0.28 29 3882 -24.95 -25.22 -25.08 0.14 29 3882 -24.87 -25.13 -25.00 0.13
19 2071 -23.54 -23.65 -23.84 -23.76 -23.68 -23.69 0.10 32D 4124 -30.05 -30.21 -30.13 0.08 32D 4124 -30.47 -30.15 -30.10 -30.24 0.16 32D 4124 -29.96 -30.02 -29.99 0.03 32D 4124 -30.80 -30.48 -30.64 0.16 32D 4124 -30.21 -29.60 -29.91 0.31 32D 4124 -29.71 -29.65 -29.68 0.03 32D 4124 -30.11 -30.52 -30.32 0.21 32D 4124 -30.83 -30.80 -30.82 0.01 32D 4124 -30.11 -29.98 -30.05 0.06 32D 4124 -30.88 -30.53 -30.71 0.18 32D 4124 -29.66 -29.77 -29.72 0.05 32D 4124 -30.13 -30.00 -30.07 0.07 32D 4124 -29.60 -30.05 -29.83 0.22 32D 4124 -30.11 -30.11 -30.11 0.00 32D 4124 -29.88 -29.79 -29.83 0.04 32D 4124 -29.96 -30.36 -30.45 -30.26 0.21 32D 4124 -30.21 -30.65 -30.43 0.22 32D 4124 -30.46 -30.99 -30.72 0.26 32D 4124 -30.86 -29.94 -30.40 0.46 32D 4124 -29.80 -30.00 -29.90 0.10
16D 2151 -30.60 -30.03 -30.18 -30.28 -30.32 -30.28 0.19 30,31 4218 -24.01 -24.05 -24.03 0.02 30,31 4218 30,31 4218 -24.91 -24.33 -24.62 0.29 30,31 4218 30,31 4218 -24.04 -23.64 -23.84 0.20 30,31 4218 -21.65 -23.82 -22.74 1.09 30,31 4218 -24.84 -24.62 -24.73 0.11 30,31 4218 30,31 4218 -24.00 -24.42 -24.21 0.21 30,31 4218 30,31 4218 -24.45 -24.30 -24.38 0.08 30,31 4218 -24.73 -25.01 -24.87 0.14 30,31 4218 -24.07 -24.64 -24.36 0.29 30,31 4218 -24.28 -23.92 -24.10 0.18 30,31 4218 -24.25 -24.38 -24.32 0.07 30,31 4218 -24.62 -24.50 -24.77 -24.63 0.11 30,31 4218 -24.65 -24.65 0.00 30,31 4218 30,31 4218 -28.20 -26.06 -27.13 1.07 30,31 4218
20 2191 -24.64 -24.92 -24.88 -24.89 -25.13 -24.89 0.16 32 4307 -24.51 -25.01 -24.76 0.25 32 4307 32 4307 -25.52 -25.75 -25.63 0.11 32 4307 32 4307 -24.20 -24.50 -24.35 0.15 32 4307 -24.19 -24.49 -24.34 0.15 32 4307 -24.75 -24.75 0.00 32 4307 32 4307 -25.11 -25.46 -25.28 0.18 32 4307 32 4307 -25.05 -25.22 -25.14 0.09 32 4307 -25.08 -24.51 -24.80 0.28 32 4307 -25.38 -25.48 -25.43 0.05 32 4307 -25.6 -24.96 -25.28 0.32 32 4307 -25.51 -24.53 -25.02 0.49 32 4307 -25.16 -25.34 -25.48 -25.33 0.13 32 4307 -25.42 -25.42 0.00 32 4307 32 4307 -25.38 -25.38 0.00 32 4307
21 2519 -23.81 -23.86 -23.90 -23.80 -23.93 -23.86 0.05
22 2535 -24.28 -24.19 -24.29 -24.36 -24.17 -24.26 0.07
19D 2625 -27.73 -27.67 -27.70 -27.72 -27.68 -27.70 0.02
IS2 2681 -29.30 -29.22 -29.41 -29.65 -29.32 -29.38 0.15
SS3 2864 -28.68 -28.54 -28.67 -28.63 -28.55 -28.62 0.06
FS3,23 2934 -27.25 -27.14 -27.24 -27.28 -27.03 -27.19 0.09
24 3008 -25.54 -25.31 -25.48 -25.83 -25.54 -25.54 0.17
PT 3097 -26.91 -26.65 -26.63 -27.17 -26.74 -26.82 0.20
24D 3284 -27.49 -27.33 -27.14 -27.22 -27.34 -27.30 0.12
25 3432 -26.93 -27.38 -27.39 -27.31 -27.07 -27.22 0.18
26 3446 -24.80 -24.42 -24.70 -24.95 -24.55 -24.68 0.19
27,28 3787 -26.64 -26.40 -26.64 -26.44 -26.36 -26.50 0.12
29 3882 -25.61 -25.20 -25.53 -25.35 -25.42 0.16
32D 4124 -30.86 -30.58 -30.67 -30.90 -30.84 -30.77 0.12
30,31 4218 -24.84 -24.72 -25.08 -24.82 -24.83 -24.86 0.12
32 4307 -26.45 -26.42 -26.21 -26.30 -26.48 -26.37 0.10
META PAH CARBON ISOTOPE DATA 12 / 05 / 03
Project #: GCIRMS Phase 2

Analyzed with Delta + XL and HP 6890 GC w/ EPC program and conditions:
GC Column : HP-5MS s/n # US3265561H 30m;025 dia.; 0.5 um film
GC Program: 40 C ( hold 1.5 min ) then 4 degrees @min to 300 ( hold 25 min.)
Injector: 300 C / splitless ( 0.8 min) purge 20 mls/min
Oxidation Reactor: 940 C

PAH AVERAGES

AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE
OU4007 GTO31010-01 GTO31010-02 GTO31010-03 GTO31010-04 GTO31010-05 GTO31010-06 GTO31010-07 GTO31010-08 GTO31010-09 GTO31010-10 GTO31010-11 GTO31010-12 GTO31010-13 GTO31010-14 GTO31010-15 GTO31010-16 GTO31010-17 GTO31010-18 GTO31010-19 GTO31010-20
3 622 -29.83 683 9D -31.22 -31.41 -31.59 -31.54 -31.40 -31.45 -31.63 -31.65 -31.46 -31.55 -30.70 -31.77 -31.52 -31.42 -31.58 -30.91 -31.58 -31.53 -31.96 -30.97
4 642 -26.72 932 10D -33.16 -32.98 -33.15 -32.99 -32.92 -32.88 -32.87 -33.09 -33.25 -33.20 -33.00 -32.96 -32.60 -32.96 -33.03 -32.82 -32.98 -33.09 -32.64 -32.86
9D 683 -29.79 1409 14 -25.53 -25.16 -25.44 -25.60 -25.35 -25.31 -25.00 -24.61
5,6 703 -27.02 1644 15 -25.76 -24.54 -24.76 -25.48 -24.83 -24.58 -24.45
7 784 -27.27 1680 16 -25.71 -26.70 -24.91 -25.96 -24.67 -24.59 -25.22
8 858 -28.02 1950 17 -26.08 -24.49 -25.07 -22.33 -21.55 -21.21 -21.23 -22.79
9 894 -27.97 2016 18 -29.82 -28.81 -25.30 -29.19 -27.17
10D 932 -32.36 2071 19 -24.10 -24.02 -24.59 -24.41 -24.41 -24.50 -24.02 -23.49 -24.18
10 957 -27.27 2151 16D -31.09 -31.10 -30.00 -31.15 -29.36 -30.11 -29.03 -30.95 -30.85 -31.08 -31.04 -30.54 -30.30 -31.14 -30.59 -29.35 -29.84 -30.77 -30.98 -29.58
11 1000 -23.48 2191 20 -23.09 -21.89 -21.21 -23.28 -21.87 -21.01 -23.61 -23.53 -24.31 -23.15 -24.98
12 1033 -32.72 2519 21 -25.08 -25.90 -25.53 -25.43 -25.05 -25.52 -25.30 -24.51 -24.71 -24.64 -24.68 -25.43 -25.75 -25.31 -25.62 -24.86 -24.98
13 1109 -26.19 2535 22 -25.16 -24.80 -24.35 -25.00 -25.00 -25.07 -24.58 -24.96 -24.55 -23.85 -24.46 -24.48 -24.84 -24.29 -24.50
14 1409 -25.20 2625 19D -27.88 -27.94 -27.25 -28.00 -28.23 -27.76 -27.18 -27.78 -27.64 -28.14 -27.54 -27.58 -27.33 -27.32 -27.60 -27.32 -27.54 -27.89 -27.71 -27.93
15 1644 -23.82 2938 23 -25.52 -28.07 -26.13 -29.81 -25.48 -25.68 -25.64 -26.16 -25.02 -25.24 -25.08 -25.29 -25.34 -25.44 -25.95 -25.71 -25.24 -24.89
FS1 1659 -23.25 3008 24 -22.55 -29.31 -25.95 -25.35 -25.12 -25.16 -23.57 -24.78 -24.80 -25.01 -24.94 -25.54 -21.92 -22.35 -25.04 -24.00
16 1680 -31.33 3284 24D -26.85 -27.12 -26.39 -27.20 -27.00 -27.39 -26.81 -27.19 -26.97 -27.58 -26.56 -26.86 -25.07 -26.74 -26.69 -26.49 -26.51 -27.16 -27.14 -26.41
SS2 1785 -31.51 3432 25 -24.46 -25.25 -24.67 -23.80 -24.23 -24.64 -23.93 -23.97 -24.15 -24.09 -24.66 -24.72 -24.40 -24.42 -24.98 -23.87
17 1950 -22.92 3446 26 -24.85 -25.27 -25.47 -24.53 -24.65 -24.48 -24.57 -24.81 -24.81 -24.98 -24.75 -25.00 -25.22 -25.33 -24.39 -24.75
FS2 1995 -26.04 3787 27,28 -24.59 -25.69 -24.55 -24.50 -24.87 -24.79 -24.67 -25.05 -24.86 -24.87 -25.02 -25.17 -25.35 -25.45 -24.68
18 2016 -24.53 3882 29 -25.88 -25.62 -26.33 -24.77 -24.98 -25.73 -25.80 -25.78 -25.81 -26.41 -25.43 -25.17 -25.30 -25.41 -24.93 -25.08 -25.00
19 2071 -23.69 4124 32D -30.13 -30.24 -29.99 -30.64 -29.91 -29.68 -30.32 -30.82 -30.05 -30.71 -29.72 -30.07 -29.83 -30.11 -29.83 -30.26 -30.43 -30.72 -30.40 -29.90
16D 2151 -30.28 4218 30,31 -24.03 -24.62 -23.84 -22.74 -24.73 -24.21 -24.38 -24.87 -24.36 -24.10 -24.32 -24.63 -24.65 -27.13
20 2191 -24.89 4307 32 -24.76 -25.63 -24.35 -24.34 -24.75 -25.28 -25.14 -24.80 -25.43 -25.28 -25.02 -25.33 -25.42 -25.38
21 2519 -23.86
22 2535 -24.26
19D 2625 -27.70
IS2 2681 -29.38
SS3 2864 -28.62
FS3,23 2934 -27.19
24 3008 -25.54
PT 3097 -26.82
24D 3284 -27.30
25 3432 -27.22 AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE AVERAGE
26 3446 -24.68 7 8 GTO31010-09 GTO31010-10 GTO31010-11 GTO31010-12 GTO31010-13 GTO31010-14 GTO31010-15 GTO31010-16 GTO31010-17 GTO31010-18 GTO31010-19 GTO31010-20
27,28 3787 -26.50 1409 14 -25.44 -25.60 -25.35 -25.31 -25.00 -24.61
29 3882 -25.42 1644 15 -24.76 -25.48 -24.83 -24.58 -24.45
32D 4124 -30.77 1680 16 -24.91 -25.96 -24.67 -24.59 -25.22
30,31 4218 -24.86 1950 17 -22.33 -21.55 -21.21 -21.23 -22.79
32 4307 -26.37 2016 18 -28.81 -25.30 -29.19 -27.17
2071 19 -24.59 -24.41 -24.41 -24.50 -24.02 -23.49 -24.18
2191 20 -23.28 -21.87 -21.01 -23.61 -23.53 -24.31 -23.15 -24.98
2519 21 -25.52 -25.30 -24.51 -24.71 -24.64 -24.68 -25.43 -25.75 -25.31 -25.62 -24.86 -24.98
2535 22 -25.00 -25.00 -25.07 -24.58 -24.96 -24.55 -23.85 -24.46 -24.48 -24.84 -24.29 -24.50
2938 23 -25.64 -26.16 -25.02 -25.24 -25.08 -25.29 -25.34 -25.44 -25.95 -25.71 -25.24 -24.89
3008 24 -25.16 -23.57 -24.78 -24.80 -25.01 -24.94 -25.54 -21.92 -22.35 -25.04 -24.00
3432 25 -24.64 -23.93 -23.97 -24.15 -24.09 -24.66 -24.72 -24.40 -24.42 -24.98 -23.87
3446 26 -24.48 -24.57 -24.81 -24.81 -24.98 -24.75 -25.00 -25.22 -25.33 -24.39 -24.75
3787 27,28 -24.87 -24.79 -24.67 -25.05 -24.86 -24.87 -25.02 -25.17 -25.35 -25.45 -24.68
3882 29 -25.73 -25.80 -25.78 -25.81 -26.41 -25.43 -25.17 -25.30 -25.41 -24.93 -25.08 -25.00
4218 30,31 -24.73 -24.21 -24.38 -24.87 -24.36 -24.10 -24.32 -24.63 -24.65 -27.13
4307 32 -24.75 -25.28 -25.14 -24.80 -25.43 -25.28 -25.02 -25.33 -25.42 -25.38
683 9D -31.63 -31.65 -31.46 -31.55 -30.70 -31.77 -31.52 -31.42 -31.58 -30.91 -31.58 -31.53 -31.96 -30.97
932 10D -32.87 -33.09 -33.25 -33.20 -33.00 -32.96 -32.60 -32.96 -33.03 -32.82 -32.98 -33.09 -32.64 -32.86
2151 16D -29.03 -30.95 -30.85 -31.08 -31.04 -30.54 -30.30 -31.14 -30.59 -29.35 -29.84 -30.77 -30.98 -29.58
2625 19D -27.18 -27.78 -27.64 -28.14 -27.54 -27.58 -27.33 -27.32 -27.60 -27.32 -27.54 -27.89 -27.71 -27.93
3284 24D -26.81 -27.19 -26.97 -27.58 -26.56 -26.86 -25.07 -26.74 -26.69 -26.49 -26.51 -27.16 -27.14 -26.41
4124 32D -30.32 -30.82 -30.05 -30.71 -29.72 -30.07 -29.83 -30.11 -29.83 -30.26 -30.43 -30.72 -30.40 -29.90

PAH AVERAGES

9D 9D 9D 9D 9D 9D 9D 9D 9D 9D 9D 9D 9D 9D 9D 9D 9D 9D 9D 9D
10D 10D 10D 10D 10D 10D 10D 10D 10D 10D 10D 10D 10D 10D 10D 10D 10D 10D 10D 10D
14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14
15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15
16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16
17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17
18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18
19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19
16D 16D 16D 16D 16D 16D 16D 16D 16D 16D 16D 16D 16D 16D 16D 16D 16D 16D 16D 16D
20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
21 21 21 21 21 21 21 21 21 21 21 21 21 21 21 21 21 21 21 21
22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22
19D 19D 19D 19D 19D 19D 19D 19D 19D 19D 19D 19D 19D 19D 19D 19D 19D 19D 19D 19D
23 23 23 23 23 23 23 23 23 23 23 23 23 23 23 23 23 23 23 23
24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24
24D 24D 24D 24D 24D 24D 24D 24D 24D 24D 24D 24D 24D 24D 24D 24D 24D 24D 24D 24D
25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25
26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28
29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29
32D 32D 32D 32D 32D 32D 32D 32D 32D 32D 32D 32D 32D 32D 32D 32D 32D 32D 32D 32D
30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31
32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32
GTO31010-01
GTO31010-02
GTO31010-03
GTO31010-04
GTO31010-05
GTO31010-06
GTO31010-07
GTO31010-08
GTO31010-09
GTO31010-10
GTO31010-11
GTO31010-12
GTO31010-13
GTO31010-14
GTO31010-15
GTO31010-16
GTO31010-17
GTO31010-18
GTO31010-19
GTO31010-20
-31.217
-31.4116666667
-31.5935
-31.5445
-31.404
-31.447
-31.632
-31.6535
-31.463
-31.5465
-30.7
-31.7735
-31.521
-31.424
-31.584
-30.91
-31.58
-31.5255
-31.9615
-30.965
-33.155
-32.9793333333
-33.1545
-32.9925
-32.92
-32.8795
-32.868
-33.0925
-33.246
-33.198
-32.995
-32.962
-32.6045
-32.962
-33.031
-32.818
-32.9805
-33.086
-32.641
-32.8603333333
-25.5305
-25.1605
-25.44
-25.598
-25.3526666667
-25.307
-24.9985
-24.61
-25.7625
-24.54
-24.763
-25.48
-24.8316666667
-24.58
-24.4473333333
-25.707
-26.7
-24.906
-25.96
-24.6746666667
-24.585
-25.2243333333
-26.08
-24.485
-25.07
-22.33
-21.55
-21.2133333333
-21.23
-22.79
-29.82
-28.81
-25.3015
-29.185
-27.165
-24.095
-24.0225
-24.5905
-24.405
-24.41
-24.4973333333
-24.015
-23.485
-24.1816666667
-31.092
-31.0986666667
-30
-31.153
-29.3555
-30.106
-29.03
-30.951
-30.8505
-31.077
-31.036
-30.539
-30.295
-31.1435
-30.5885
-29.3533333333
-29.835
-30.7735
-30.9815
-29.5783333333
-23.085
-21.89
-21.21
-23.275
-21.87
-21.01
-23.6066666667
-23.53
-24.309
-23.15
-24.9766666667
-25.0825
-25.895
-25.529
-25.4255
-25.05
-25.52
-25.301
-24.506
-24.712
-24.644
-24.681
-25.425
-25.752
-25.3123333333
-25.616
-24.8555
-24.9806666667
-25.1625
-24.8
-24.3495
-24.9955
-24.995
-25.07
-24.576
-24.955
-24.545
-23.854
-24.455
-24.4826666667
-24.8355
-24.289
-24.4986666667
-27.8835
-27.9416666667
-27.245
-28.0005
-28.234
-27.756
-27.175
-27.783
-27.64
-28.14
-27.54
-27.578
-27.325
-27.32
-27.595
-27.3166666667
-27.535
-27.8925
-27.712
-27.93
-25.5185
-28.065
-26.1295
-29.81
-25.475
-25.68
-25.6365
-26.159
-25.016
-25.236
-25.084
-25.29
-25.343
-25.4375
-25.9466666667
-25.7085
-25.2435
-24.89
-22.545
-29.305
-25.9475
-25.35
-25.115
-25.1585
-23.565
-24.7815
-24.798
-25.014
-24.9445
-25.5405
-21.9233333333
-22.35
-25.0415
-24.003
-26.852
-27.1185
-26.385
-27.195
-26.995
-27.385
-26.81
-27.187
-26.974
-27.5785
-26.56
-26.855
-25.0705
-26.74
-26.69
-26.4933333333
-26.51
-27.162
-27.14
-26.4133333333
-24.4575
-25.245
-24.665
-23.8025
-24.233
-24.64
-23.9255
-23.97
-24.147
-24.087
-24.6575
-24.721
-24.4
-24.415
-24.984
-23.8665
-24.85
-25.271
-25.47
-24.5335
-24.6535
-24.4845
-24.565
-24.81
-24.8115
-24.98
-24.7545
-25.001
-25.2166666667
-25.33
-24.3865
-24.75
-24.59
-25.6855
-24.5455
-24.496
-24.8655
-24.7865
-24.6655
-25.0505
-24.857
-24.8685
-25.0195
-25.1693333333
-25.3495
-25.448
-24.6775
-25.882
-25.622
-26.325
-24.7725
-24.9795
-25.725
-25.795
-25.78
-25.81
-26.41
-25.426
-25.1735
-25.3
-25.41
-24.925
-25.081
-25
-30.13
-30.24
-29.99
-30.64
-29.905
-29.68
-30.315
-30.817
-30.045
-30.705
-29.715
-30.065
-29.825
-30.1085
-29.8335
-30.2566666667
-30.43
-30.7245
-30.397
-29.9015
-24.03
-24.62
-23.84
-22.735
-24.73
-24.21
-24.375
-24.87
-24.355
-24.0995
-24.315
-24.63
-24.65
-27.1305
-24.762
-25.6325
-24.35
-24.3375
-24.75
-25.283
-25.135
-24.795
-25.43
-25.28
-25.02
-25.3266666667
-25.42
-25.377

Sheet3

7
8
Peak ID
Carbon isotope value

08

PAHs-Combined GC and GCIRMS Data

08

02

Naphthalene

Benzo(G,H,I) perylene

Acenaphthalene

Pyrene

Chart1

14 14
15 15
16 16
17 17
18 18
19 19
20 20
21 21
22 22
23 23
24 24
25 25
26 26
27,28 27,28
29 29
30,31 30,31
32 32
9D 9D
10D 10D
16D 16D
19D 19D
24D 24D
32D 32D
02DF
08DF
Peak ID
C Isotope value
-19.695
-20.54
-22.147
-32.115
-21.659
-22.81
-31.266
-23.035
-35.21
-22.256
-27.455
-22.52
-25.625
-22.5645
-25.665
-22.73
-31.1
-24.1295
-28.3
-23.541
-35.4175
-31.3195
-31.305
-32.876
-32.9625
-30.705
-30.664
-27.094
-28.3925
-26.8375
-26.9915
-29.741
-29.735

PAH DATA

1119 1135 1149 Average Stdevp 1120 1132 Average Stdevp 1121 1136 Average Stdevp 1122 1137 Average Stdevp 1124 1139 Average Stdevp 1127 1140 Average Stdevp 1128 1141 Average Stdevp 1125 1142 Average Stdevp 1126 1143 Average Stdevp 1131 1145 Average Stdevp
OU4007 OU4007 OU4007 OU4007 OU4007 IG011003-01DF IG011003-01DF IG011003-01DF IG011003-01DF IG011003-02DF IG011003-02DF IG011003-02DF IG011003-02DF IG011003-04DF IG011003-04DF IG011003-04DF IG011003-04DF IGO11003-06DF IGO11003-06DF IGO11003-06DF IGO11003-06DF IG011003-08DF IG011003-08DF IG011003-08DF IG011003-08DF IG011003-10DF IG011003-10DF IG011003-10DF IG011003-10DF IG011003-11DF IG011003-11DF IG011003-11DF IG011003-11DF IGO11003-17DF IGO11003-17DF IGO11003-17DF IGO11003-17DF IG011003-18DF IG011003-18DF IG011003-18DF IG011003-18DF
3 602 -28.44 -28.39 -28.38 -28.40 0.03 660 9D -31.13 -31.39 -31.26 0.13 660 9D -31.37 -31.27 -31.32 0.05 660 9D -31.41 -31.40 -31.40 0.00 660 9D -31.37 -31.20 -31.28 0.08 660 9D -31.24 -31.37 -31.31 0.06 660 9D -31.16 -31.30 -31.23 0.07 660 9D -31.35 -31.17 -31.26 0.09 660 9D -31.32 -31.56 -31.44 0.12 660 9D -31.19 -31.02 -31.11 0.09
4 622 -26.71 -26.68 -26.89 -26.76 0.09 900 10D -33.00 -33.17 -33.09 0.09 900 10D -32.79 -32.96 -32.88 0.09 900 10D -32.57 -33.00 -32.78 0.22 900 10D -33.01 -32.41 -32.71 0.30 900 10D -32.90 -33.03 -32.96 0.06 900 10D -32.81 -33.14 -32.97 0.17 900 10D -33.13 -32.70 -32.92 0.21 900 10D -33.11 -33.14 -33.13 0.01 900 10D -32.87 -32.95 -32.91 0.04
9D 660 -28.80 -28.25 -28.51 -28.52 0.22 1369 14 1369 14 -21.02 -18.37 -19.70 1.32 1369 14 -24.99 -25.25 -25.12 0.13 1369 14 -21.40 -21.65 -21.53 0.13 1369 14 1369 14 -23.22 1369 14 -22.42 -22.36 -22.39 0.03 1369 14 -24.90 -24.86 -24.88 0.02 1369 14 -24.50 -24.33 -24.42 0.08
5,6 679 -27.35 -27.50 -27.40 -27.41 0.06 1603 15 1603 15 1603 15 1603 15 1603 15 1603 15 1603 15 -26.08 -26.76 -26.42 0.34 1603 15 1603 15 -24.84 -24.67 -24.75 0.09
7 756 -26.84 -26.86 -27.35 -27.01 0.24 1639 16 1639 16 1639 16 1639 16 1639 16 1639 16 1639 16 -26.35 -27.32 -26.84 0.49 1639 16 1639 16 -24.22 -23.89 -24.06 0.17
8 827 -27.82 -27.98 -28.51 -28.10 0.30 1908 17 -24.00 -21.35 -22.68 1.33 1908 17 -21.07 -20.01 -20.54 0.53 1908 17 -28.19 -28.19 0.00 1908 17 -20.77 -20.27 -20.52 0.25 1908 17 1908 17 1908 17 -24.11 -23.45 -23.78 0.33 1908 17 -25.58 -24.80 -25.19 0.39 1908 17 -21.08 -20.06 -20.57 0.51
9 863 -27.15 -27.17 -27.95 -27.42 0.37 1974 18 1974 18 1974 18 1974 18 1974 18 1974 18 1974 18 -27.78 -26.91 -27.34 0.43 1974 18 -26.94 -26.48 -26.71 0.23 1974 18
10D 900 -32.40 -32.36 -32.55 -32.44 0.08 2029 19 2029 19 2029 19 2029 19 2029 19 2029 19 2029 19 -27.56 -27.56 0.00 2029 19 -24.09 -23.94 -24.02 0.08 2029 19 -22.13 -21.93 -22.03 0.10
10 924 -26.86 -27.31 -27.45 -27.21 0.25 2113 16D -30.57 -29.82 -30.20 0.38 2113 16D -30.85 -30.56 -30.71 0.15 2113 16D -30.76 -30.52 -30.64 0.12 2113 16D -30.15 -30.25 -30.20 0.05 2113 16D -30.57 -30.76 -30.66 0.10 2113 16D -30.99 -31.15 -31.07 0.08 2113 16D -29.06 -29.67 -29.36 0.31 2113 16D -30.58 -30.88 -30.73 0.15 2113 16D -29.63 -29.59 -29.61 0.02
11 966 -23.36 -23.27 -24.19 -23.61 0.41 2148 20 2148 20 2148 20 2148 20 2148 20 2148 20 2148 20 -26.15 -25.66 -25.91 0.24 2148 20 -31.92 -32.60 -32.26 0.34 2148 20 -22.62 -22.21 -22.42 0.21
12 999 -32.52 -32.71 -33.98 -33.07 0.65 2475 21 -22.22 -21.58 -21.90 0.32 2475 21 -22.25 -22.04 -22.15 0.10 2475 21 -25.82 -25.28 -25.55 0.27 2475 21 -21.80 -20.91 -21.35 0.45 2475 21 -32.41 -31.82 -32.12 0.30 2475 21 -24.76 -25.47 -25.12 0.35 2475 21 -26.16 -25.88 -26.02 0.14 2475 21 -24.88 -24.78 -24.83 0.05 2475 21 -22.80 -22.54 -22.67 0.13
13 1073 -25.84 -25.75 -26.96 -26.18 0.55 2491 22 -23.92 -24.48 -24.20 0.28 2491 22 -21.24 -22.08 -21.66 0.42 2491 22 2491 22 -23.63 -25.39 -24.51 0.88 CE 2491 22 2491 22 2491 22 2491 22 -25.32 -25.38 -25.35 0.03 2491 22 -26.16 -25.95 -26.06 0.10
14 1369 -24.66 -24.74 -25.28 -24.89 0.27 2587 19D -27.23 -27.00 -27.12 0.11 2587 19D -26.99 -27.20 -27.09 0.10 2587 19D -27.85 -27.78 -27.81 0.03 2587 19D -26.67 -27.00 -26.83 0.17 2587 19D -28.98 -27.80 -28.39 0.59 2587 19D -29.67 -29.01 -29.34 0.33 2587 19D -27.49 -26.70 -27.10 0.40 2587 19D -27.85 -27.66 -27.76 0.09 2587 19D -27.31 -27.04 -27.17 0.13
15 1603 -22.71 -22.67 -24.22 -23.20 0.72 2899 23 -22.93 -22.97 -22.95 0.02 2899 23 -23.06 -22.56 -22.81 0.25 2899 23 -25.00 -24.73 -24.87 0.13 2899 23 -22.80 -22.21 -22.51 0.29 2899 23 -31.60 -30.93 -31.27 0.33 2899 23 -26.13 -26.51 -26.32 0.19 2899 23 2899 23 -26.13 -25.98 -26.06 0.07 2899 23 -23.13 -23.06 -23.10 0.03
FS1 1618 -24.18 -25.70 -25.22 -25.03 0.63 2964 24 -22.84 -23.09 -22.96 0.13 2964 24 -22.99 -23.08 -23.04 0.05 2964 24 -24.42 -24.29 -24.36 0.07 2964 24 -22.80 -22.55 -22.67 0.12 2964 24 -35.48 -34.94 -35.21 0.27 2964 24 -24.58 -24.66 -24.62 0.04 2964 24 -26.62 -26.37 -26.50 0.12 2964 24 -26.49 -26.46 -26.47 0.01 2964 24 -24.18 -23.75 -23.96 0.22
16 1639 -30.65 -31.06 -30.00 -30.57 0.44 3246 24D -26.20 -26.34 -26.27 0.07 3246 24D -26.83 -26.85 -26.84 0.01 3246 24D -27.33 -27.13 -27.23 0.10 3246 24D -26.64 -26.83 -26.74 0.09 3246 24D -27.01 -26.97 -26.99 0.02 3246 24D -27.02 -27.23 -27.12 0.10 3246 24D -27.08 -26.23 -26.66 0.43 3246 24D -27.31 -26.78 -27.05 0.26 3246 24D -26.51 -26.26 -26.39 0.12
SS2 1744 -31.50 -31.08 -31.51 -31.36 0.20 3388 25 -22.36 -22.44 -22.40 0.04 3388 25 -22.06 -22.45 -22.26 0.19 3388 25 -25.30 -25.22 -25.26 0.04 3388 25 -23.55 -23.38 -23.47 0.09 3388 25 -27.81 -27.10 -27.46 0.36 3388 25 -24.79 -25.25 -25.02 0.23 3388 25 3388 25 -26.87 -27.14 -27.01 0.13 3388 25 -25.27 -24.86 -25.07 0.21
17 1908 -21.45 -21.15 -22.80 -21.80 0.72 3402 26 -22.72 -22.90 -22.81 0.09 3402 26 -22.48 -22.56 -22.52 0.04 3402 26 -23.48 -23.22 -23.35 0.13 3402 26 -22.12 -22.68 -22.40 0.28 3402 26 -25.86 -25.39 -25.63 0.24 3402 26 -23.53 -23.38 -23.45 0.08 3402 26 3402 26 -24.31 -24.31 0.00 3402 26 -24.05 -23.75 -23.90 0.15
FS2 1953 -25.39 -26.15 -31.36 -27.63 2.65 3751 27,28 -23.02 -23.14 -23.08 0.06 3751 27,28 -22.32 -22.81 -22.56 0.25 3751 27,28 -24.04 -24.51 -24.28 0.24 3751 27,28 -22.36 -22.52 -22.44 0.08 3751 27,28 -25.68 -25.65 -25.67 0.02 3751 27,28 -24.59 -24.63 -24.61 0.02 3751 27,28 3751 27,28 -25.12 -25.01 -25.07 0.06 3751 27,28 -24.00 -23.83 -23.92 0.09
18 1974 -24.34 -24.46 -23.22 -24.01 0.56 3837 29 -23.05 -23.04 -23.04 0.00 3837 29 -22.49 -22.97 -22.73 0.24 3837 29 -23.87 -24.12 -24.00 0.13 3837 29 -22.73 -22.76 -22.75 0.02 3837 29 -31.25 -30.95 -31.10 0.15 3837 29 -25.04 -24.91 -24.98 0.06 3837 29 3837 29 -25.68 -26.00 -25.84 0.16 3837 29 -24.57 -24.51 -24.54 0.03
19 2029 -23.30 -23.55 -23.85 -23.57 0.22 4080 32D -29.05 -29.06 -29.05 0.01 4080 32D -29.85 -29.63 -29.74 0.11 4080 32D -29.82 -29.75 -29.79 0.03 4080 32D -29.82 -29.98 -29.90 0.08 4080 32D -29.53 -29.94 -29.74 0.21 4080 32D -29.67 -29.80 -29.74 0.07 4080 32D -29.80 -29.65 -29.73 0.07 4080 32D -30.18 -29.76 -29.97 0.21 4080 32D -29.61 -29.36 -29.48 0.12
16D 2113 -30.58 -30.27 -30.46 -30.44 0.13 4173 30,31 -22.87 -22.86 -22.86 0.00 4173 30,31 -24.32 -23.94 -24.13 0.19 4173 30,31 -25.43 -25.39 -25.41 0.02 4173 30,31 -22.62 -22.95 -22.79 0.17 4173 30,31 -28.27 -28.33 -28.30 0.03 4173 30,31 -25.45 -25.19 -25.32 0.13 4173 30,31 4173 30,31 4173 30,31 -24.04 -23.94 -23.99 0.05
20 2148 -24.40 -25.32 -26.23 -25.32 0.75 4244 32 -23.71 -23.87 -23.79 0.08 4244 32 -23.74 -23.35 -23.54 0.20 4244 32 -25.19 -24.89 -25.04 0.15 4244 32 -23.26 -23.56 -23.41 0.15 4244 32 -35.64 -35.20 -35.42 0.22 4244 32 -25.47 -25.46 -25.46 0.01 4244 32 4244 32 4244 32 -24.53 -24.21 -24.37 0.16
21 2475 -23.88 -23.40 -24.91 -24.06 0.63
22 2491 -23.57 -24.96 -23.30 -23.94 0.73
19D 2587 -27.57 -27.17 -27.54 -27.42 0.18
IS2 2639 -29.16 -29.43 -29.91 -29.50 0.31
SS3 2821 -28.30 -28.30 -28.65 -28.42 0.17
FS3,23 2899 -26.13 -26.77 -26.90 -26.60 0.34
24 2964 -25.31 -25.94 -25.25 -25.50 0.31
PT 3055 -26.10 -26.31 -26.76 -26.39 0.27
24D 3246 -26.69 -26.56 -26.89 -26.71 0.14
25 3388 -26.30 -25.76 -27.70 -26.59 0.82
26 3402 -24.02 -25.08 -24.22 -24.44 0.46
27,28 3751 -25.77 -25.92 -26.36 -26.02 0.25
29 3837 -24.64 -25.07 -24.81 -24.84 0.18
32D 4080 -29.59 -29.55 -29.60 -29.58 0.02
30,31 4173 -24.00 -24.40 -24.07 -24.16 0.17
32 4244 -25.76 -26.01 -25.50 -25.76 0.21
META PAH CARBON ISOTOPE DATA 01 / 07 / 04
Project # : LAMPBLACK

Analyzed with Delta + XL and HP 6890 GC w/ EPC program and conditions:
GC Column : HP-5MS s/n # US3265561H 30m;025 dia.; 0.5 um film
GC Program: 40 C ( hold 1.5 min ) then 4 degrees @min to 300 ( hold 25 min.)
Injector: 300 C / splitless ( 0.8 min) purge 20 mls/min
Oxidation Reactor: 940 C

PAH AVERAGES

Average Average Average Average Average Average Average
OU4007 IG011003-02DF IG011003-08DF IG011003-10DF IG011003-11DF IGO11003-17DF IG011003-18DF
3 602 -28.40 660 9D -31.32 -31.31 -31.23 -31.26 -31.44 -31.11
4 622 -26.76 900 10D -32.88 -32.96 -32.97 -32.92 -33.13 -32.91
9D 660 -28.52 1369 14 -19.70 -22.39 -24.88 -24.42
5,6 679 -27.41 1603 15 -26.42 -24.75
7 756 -27.01 1639 16 -26.84 -24.06
8 827 -28.10 1908 17 -20.54 -23.78 -25.19 -20.57
9 863 -27.42 1974 18 -27.34 -26.71
10D 900 -32.44 2029 19 -27.56 -24.02 -22.03
10 924 -27.21 2113 16D -30.71 -30.66 -31.07 -29.36 -30.73 -29.61
11 966 -23.61 2148 20 -25.91 -32.26 -22.42
12 999 -33.07 2475 21 -22.15 -32.12 -25.12 -26.02 -24.83 -22.67
13 1073 -26.18 2491 22 -21.66 -25.35 -26.06
14 1369 -24.89 2587 19D -27.09 -28.39 -29.34 -27.10 -27.76 -27.17
15 1603 -23.20 2899 23 -22.81 -31.27 -26.32 -26.06 -23.10
FS1 1618 -25.03 2964 24 -23.04 -35.21 -24.62 -26.50 -26.47 -23.96
16 1639 -30.57 3246 24D -26.84 -26.99 -27.12 -26.66 -27.05 -26.39
SS2 1744 -31.36 3388 25 -22.26 -27.46 -25.02 -27.01 -25.07
17 1908 -21.80 3402 26 -22.52 -25.63 -23.45 -24.31 -23.90
FS2 1953 -27.63 3751 27,28 -22.56 -25.67 -24.61 -25.07 -23.92
18 1974 -24.01 3837 29 -22.73 -31.10 -24.98 -25.84 -24.54
19 2029 -23.57 4080 32D -29.74 -29.74 -29.74 -29.73 -29.97 -29.48
16D 2113 -30.44 4173 30,31 -24.13 -28.30 -25.32 -23.99
20 2148 -25.32 4244 32 -23.54 -35.42 -25.46 -24.37
21 2475 -24.06
22 2491 -23.94
19D 2587 -27.42
IS2 2639 -29.50
SS3 2821 -28.42
FS3,23 2899 -26.60
24 2964 -25.50
PT 3055 -26.39
24D 3246 -26.71
25 3388 -26.59
26 3402 -24.44
27,28 3751 -26.02
29 3837 -24.84
32D 4080 -29.58
30,31 4173 -24.16
32 4244 -25.76
Average Average Average Average Average Average
02DF 08DF IG011003-10DF IG011003-11DF IGO11003-17DF IG011003-18DF
1369 14 -19.70 -22.39 -24.88 -24.42
1603 15 -26.42 -24.75
1639 16 -26.84 -24.06
1908 17 -20.54 -23.78 -25.19 -20.57
1974 18 -27.34 -26.71
2029 19 -27.56 -24.02 -22.03
2148 20 -25.91 -32.26 -22.42
2475 21 -22.15 -32.12 -25.12 -26.02 -24.83 -22.67
2491 22 -21.66 -25.35 -26.06
2899 23 -22.81 -31.27 -26.32 -26.06 -23.10
2964 24 -23.04 -35.21 -24.62 -26.50 -26.47 -23.96
3388 25 -22.26 -27.46 -25.02 -27.01 -25.07
3402 26 -22.52 -25.63 -23.45 -24.31 -23.90
3751 27,28 -22.56 -25.67 -24.61 -25.07 -23.92
3837 29 -22.73 -31.10 -24.98 -25.84 -24.54
4173 30,31 -24.13 -28.30 -25.32 -23.99
4244 32 -23.54 -35.42 -25.46 -24.37
660 9D -31.32 -31.31 -31.23 -31.26 -31.44 -31.11
900 10D -32.88 -32.96 -32.97 -32.92 -33.13 -32.91
2113 16D -30.71 -30.66 -31.07 -29.36 -30.73 -29.61
2587 19D -27.09 -28.39 -29.34 -27.10 -27.76 -27.17
3246 24D -26.84 -26.99 -27.12 -26.66 -27.05 -26.39
4080 32D -29.74 -29.74 -29.74 -29.73 -29.97 -29.48

PAH AVERAGES

14 14 14 14 14 14 14 14 14
15 15 15 15 15 15 15 15 15
16 16 16 16 16 16 16 16 16
17 17 17 17 17 17 17 17 17
18 18 18 18 18 18 18 18 18
19 19 19 19 19 19 19 19 19
20 20 20 20 20 20 20 20 20
21 21 21 21 21 21 21 21 21
22 22 22 22 22 22 22 22 22
23 23 23 23 23 23 23 23 23
24 24 24 24 24 24 24 24 24
25 25 25 25 25 25 25 25 25
26 26 26 26 26 26 26 26 26
27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28 27,28
29 29 29 29 29 29 29 29 29
30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31 30,31
32 32 32 32 32 32 32 32 32
9D 9D 9D 9D 9D 9D 9D 9D 9D
10D 10D 10D 10D 10D 10D 10D 10D 10D
16D 16D 16D 16D 16D 16D 16D 16D 16D
19D 19D 19D 19D 19D 19D 19D 19D 19D
24D 24D 24D 24D 24D 24D 24D 24D 24D
32D 32D 32D 32D 32D 32D 32D 32D 32D
IG011003-01DF
IG011003-02DF
IG011003-04DF
IGO11003-06DF
IG011003-08DF
IG011003-10DF
IG011003-11DF
IGO11003-17DF
IG011003-18DF
-19.695
-25.121
-21.525
-22.39
-24.88
-24.415
-26.4175
-24.7535
-26.835
-24.055
-22.675
-20.54
-28.192
-20.5195
-23.779
-25.19
-20.57
-27.3445
-26.71
-27.562
-24.015
-22.03
-25.905
-32.2585
-22.415
-21.898
-22.147
-25.5515
-21.353
-32.115
-25.115
-26.022
-24.83
-22.668
-24.1985
-21.659
-24.51
-25.35
-26.055
-22.949
-22.81
-24.867
-22.5065
-31.266
-26.3195
-26.056
-23.095
-22.963
-23.035
-24.355
-22.6725
-35.21
-24.619
-26.4955
-26.4735
-23.964
-22.4015
-22.256
-25.26
-23.465
-27.455
-25.02
-27.007
-25.065
-22.8095
-22.52
-23.35
-22.402
-25.625
-23.4525
-24.312
-23.9
-23.0775
-22.5645
-24.275
-22.44
-25.665
-24.608
-25.065
-23.915
-23.044
-22.73
-23.995
-22.745
-31.1
-24.975
-25.84
-24.5405
-22.863
-24.1295
-25.411
-22.7855
-28.3
-25.32
-23.99
-23.789
-23.541
-25.0415
-23.4105
-35.4175
-25.4635
-24.3675
-31.262
-31.3195
-31.4025
-31.2845
-31.305
-31.231
-31.257
-31.435
-31.1065
-33.085
-32.876
-32.7805
-32.712
-32.9625
-32.974
-32.915
-33.1255
-32.909
-30.195
-30.705
-30.6415
-30.2015
-30.664
-31.0685
-29.3635
-30.7275
-29.6135
-27.116
-27.094
-27.814
-26.8345
-28.3925
-29.3405
-27.095
-27.755
-27.173
-26.27
-26.8375
-27.228
-26.737
-26.9915
-27.1235
-26.657
-27.045
-26.3855
-29.05
-29.741
-29.785
-29.901
-29.735
-29.735
-29.725
-29.97
-29.4845

Sheet3

02DF
08DF
Peak ID
C Isotope value

CSIRs of NAPL Samples

*

GC conditions

META PAH Samples 10 / 02

GC and column conditions :
Column: Restek RTX-5 / 30m, 0.25 dia., 0.50 um film
Column flow: 1.4 mls/min
Injector: 310 C / Splitless
GC program:
40C ( hold 1.5m) 4 deg. @min. to 300 ; hold 25 min. / 90 min. total

Carbon Combustion Interface: 940 C

lab dups MT07

nap nap nap nap
mn2 mn2 mn2 mn2
mn1 mn1 mn1 mn1
acy acy acy acy
ace ace ace ace
dba dba dba dba
flu flu flu flu
phe phe phe phe
ant ant ant ant
fly fly fly fly
pyr pyr pyr pyr
baa baa baa baa
chr chr chr chr
bbkf bbkf bbkf bbkf
bap bap bap bap
ip_dba ip_dba ip_dba ip_dba
bp bp bp bp
ILMT07
ILMT07 R1
ILMT07 R2
ILMT07 AVE
Compound
Isotope ratio
-25.63
-25.63
-25.43
-24.99
-27.42
-24.28
-26.69
-24.03
-23.97
-24.6666666667
-24.78
-24.64
-24.6955555556
-26.24
-25.46
-25.5
-25.7333333333
-25.67
-25.1633333333
-25.53
-25.4544444444
-25.6833333333
-25.16
-25.45
-25.4311111111
-24.27
-24.49
-24.69
-24.4833333333
-26.31
-24.9466666667
-25.74
-25.6655555556
-28.46
-27.78
-28.78
-28.34
-25.42
-26.19
-26.24
-26.03
-26.2
-27.76
-21.76
-23.76

PAH data

Average Average Average Average Average Average Average Average Average Average Average Average Average Average Average Average Average Average
Peak# Name OU 4007 Ical T-185 ER011212-02 DF ER011212-03AVE ER011212-07 DF ER011212-14 DF ER011212-14 DUP 1DF ER011212-14DUP 2DF ER011212-14AVE ER011212-16DF ER011212-17 DF ER011212-151 DF ER11115-02 DF ER11115-03 DF GT020702-01 DF GTO020719-03 DF ER020827-01 DUP 1DF ER020827-01 DUP 2DF ER020827-01AVE ER020827-02 DF GT020828-01 DF
ICAL T185
3 eb -28.41
4 m/p-x -27.00 -23.33
5,6 ox_sty -27.08 -23.61
7 ipt -27.08
8 pb -28.03
9 tmb135 -27.47
10 tmb124 -26.88 -20.54
11 sbb -23.47
12 pipt -32.83
13 nbb -25.96
14 nap -24.91 -24.24 -25.63 -25.63 -24.42 -27.17
15 mn2 -22.47 -23.30 -23.84 -25.43 -24.99 -27.42 -23.98 -24.40 -27.94 -28.41 -24.59
16 mn1 -30.25 -23.42 -24.18 -24.28 -26.69 -23.44 -27.85 -28.02 -24.59
17 acy -21.93 -23.59 -25.10 -24.73 -22.62 -26.72 -23.95
18 ace -24.04 -22.81 -24.08 -27.95 -24.84
19 dba -23.39 -22.63 -22.23 -24.03 -23.97 -23.60 -23.60 -26.08 -23.41
20 flu -24.23 -24.44 -23.51 -25.67 -22.46 -27.52 -28.62 -24.68
21 phe -23.37 -24.65 -24.09 -24.66 -24.67 -24.78 -24.64 -24.70 -23.65 -23.68 -24.45 -24.19 -24.04 -27.46 -28.60 -24.19 -24.69
22 ant -23.57 -25.45 -25.67 -26.24 -25.46 -25.50 -25.73 -24.77 -26.64 -27.30 -29.03 -25.93 -25.73
23 fly -24.45 -24.45 -24.90 -25.03 -26.20 -25.67 -25.16 -25.53 -25.45 -24.33 -24.12 -24.99 -25.01 -24.39 -27.08 -28.99 -24.91 -24.30 -24.60 -25.17 -25.30
24 pyr -24.88 -24.54 -23.95 -25.26 -24.07 -25.68 -25.16 -25.45 -25.43 -23.22 -23.66 -24.77 -24.99 -24.84 -27.32 -29.48 -24.71 -25.05 -24.84 -24.80 -24.84
25 baa -25.96 -23.81 -23.22 -24.29 -22.81 -24.27 -24.49 -24.69 -24.48 -23.04 -23.00 -23.12 -23.76 -22.45 -26.60 -27.47 -23.71 -23.43 -24.19 -23.98
26 chr -24.20 -24.80 -24.12 -25.18 -25.12 -26.31 -24.95 -25.74 -25.67 -23.59 -24.27 -24.37 -24.95 -23.54 -26.80 -28.16 -25.46 -24.90 -24.98 -25.85 -25.29
27,28 bbkf -25.68 -24.45 -26.33 -26.17 -33.41 -28.46 -27.78 -28.78 -28.34 -31.87 -28.27 -32.77 -29.69 -31.01 -26.14 -27.83 -28.61 -25.94 -31.83 -27.17 -24.34
29 bap -24.66 -24.63 -23.82 -25.68 -26.04 -25.42 -26.19 -26.24 -26.03 -25.47 -24.64 -29.92 -26.37 -26.66 -27.08 -29.13 -26.42 -27.42 -27.25 -26.13
30,31 ip_dba -24.03 -23.37 -29.67 -25.27 -26.20 -27.76 -26.88 -26.30 -24.72 -25.26 -30.63 -23.07 -23.69
32 bp -26.08 -26.22 -23.58 -24.48 -20.81 -21.76 -23.76 -22.59 -24.03 -22.14 -23.75 -25.17 -26.48 -22.68 -21.42 -20.60 -27.87 -25.54
9D -30.99 -31.53 -31.49 -31.42 -32.52 -31.21 -31.68 -31.38 -31.42 -31.47 -31.52 -31.63 -31.07 -31.59 -31.13 -31.01 -31.30 -32.21 -31.75 -31.52 -31.18
10D -32.54 -32.74 -32.81 -32.80 -32.75 -32.63 -33.24 -32.59 -32.82 -32.42 -32.89 -33.05 -32.69 -32.65 -32.33 -32.22 -32.47 -33.41 -32.94 -32.99 -32.52
16D -30.47 -30.89 -30.86 -30.46 -30.68 -30.62 -30.62 -30.76 -30.67 -30.14 -29.98 -30.60 -31.06 -30.50 -31.16 -29.74 -30.37 -30.31 -30.34 -31.00 -30.87
19D -27.46 -27.27 -27.44 -27.03 -27.75 -27.40 -27.37 -27.24 -27.34 -27.13 -26.97 -27.53 -27.14 -27.53 -27.00 -27.45 -27.32 -27.46 -27.39 -28.77 -27.55
24D -26.42 -26.83 -26.12 -26.69 -26.71 -26.68 -26.94 -26.82 -26.81 -26.43 -26.37 -26.88 -26.98 -26.85 -26.53 -26.25 -26.58 -26.75 -26.66 -26.79 -26.73
32D -29.36 -29.52 -28.27 -29.44 -29.34 -29.77 -29.10 -29.35 -29.41 -28.60 -29.35 -29.64 -29.84 -29.81 -29.44 -29.51 -29.65 -30.15 -29.90 -30.12 -29.84
SS2 2FBP -31.09 -30.89 -31.86 -31.30 -31.52 -31.60 -31.50 -31.43 -31.51 -31.11 -30.76 -31.34 -31.71 -31.46 -31.08 -31.14 -31.13 -31.33 -31.23 -31.91 -31.39
IS2 OTP -29.08
SS3 5AA -28.27 -27.18
PT PTP -25.95 -24.44 -25.55 -26.05 -26.56 -26.67 -26.34 -26.49 -26.50 -25.60 -25.60 -26.19 -27.39 -26.38 -25.96 -26.69 -26.24 -26.47 -26.36 -26.82 -26.33
FS1 dbt -25.58 -24.45
FS2 bn -24.95 -25.08 -27.91 -25.04 -25.98 -24.88 -26.90 -25.99 -25.92 -23.42 -24.26 -25.39 -24.87 -26.11 -24.05 -27.50 -25.40 -26.45 -25.90 -24.67
FS3 cod -28.78 -25.52 -27.25 -26.01 -26.81
&L&P&R&F&A

Chart1

-30.89
-30.86
-31.3
-30.675
-31.3
-30.1433333333
-29.98
-30.6033333333
-31.055
-30.5
-31.1633333333
-29.7366666667
-30.33
-31.0033333333
-30.87
-30.4671428571
16D

DA

nap nap nap nap
mn2 mn2 mn2 mn2
mn1 mn1 mn1 mn1
acy acy acy acy
ace ace ace ace
dbf dbf dbf dbf
flu flu flu flu
phe phe phe phe
ant ant ant ant
fly fly fly fly
pyr pyr pyr pyr
baa baa baa baa
chr chr chr chr
bbkf bbkf bbkf bbkf
bap bap bap bap
ip_dba ip_dba ip_dba ip_dba
bp bp bp bp
DA10
DA09
DA05
DA NAPL
Compound
δ13C (‰)
-24.24
-23.84
-24.5933333333
-24.18
-24.5933333333
-25.1
-23.9466666667
-24.08
-24.84
-22.23
-23.41
-23.51
-25.67
-24.6766666667
-24.06
-24.09
-24.45
-24.6866666667
-25.67
-26.05
-24.77
-25.73
-25.03
-24.9
-24.99
-25.3033333333
-25.26
-23.95
-24.77
-24.84
-24.29
-23.22
-23.12
-23.98
-25.18
-24.12
-24.37
-25.2933333333
-26.17
-26.33
-24.3366666667
-25.68
-23.815
-26.13
-25.27
-26.88
-23.69
-24.48
-23.58
-22.14
-25.54

PAH data (2)

Average Average Average Average Average Average Average Average Average Average Average Average Average
Peak# Name T-185 ER011212-02 DF ER011212-03AVE ER011212-07 DF ER011212-14AVE ER011212-16DF ER011212-17 DF ER011212-151 DF ER11115-02 DF ER11115-03 DF GT020702-01 DF GTO020719-03 DF ER020827-01AVE ER020827-02 DF GT020828-01 DF OU 4007 Ical
T185 DA09 DA10 MT03 MT07 MT09 MT10 DA05 RF08 RF09 RF NAPL MT NAPL RF01 RF06 DA NAPL ICAL
3 eb -28.41
4 m/p-x -23.33 -27.00
5,6 ox_sty -23.61 -27.08
7 ipt -27.08
8 pb -28.03
9 tmb135 -27.47
10 tmb124 -20.54 -26.88
11 sbb -23.47
12 pipt -32.83
13 nbb -25.96
14 nap -24.24 -25.63 -24.42 -24.19 -27.17 -24.91
15 mn2 -23.30 -23.84 -24.99 -23.98 -24.40 -27.94 -28.41 -24.59 -22.47
16 mn1 -23.42 -24.18 -24.28 -23.44 -27.85 -28.02 -24.59 -30.25
17 acy -23.59 -25.10 -24.73 -22.62 -26.72 -23.95 -21.93
18 ace -22.81 -24.08 -27.95 -24.84 -24.04
19 dbf -22.63 -22.23 -24.03 -23.60 -23.60 -26.08 -23.41 -23.39
20 flu -24.44 -23.51 -25.67 -22.46 -27.52 -28.62 -24.68 -24.23
21 phe -24.65 -24.09 -24.06 -24.53 -24.64 -23.65 -23.68 -24.45 -24.19 -24.04 -27.46 -28.60 -24.19 -24.69 -23.37
22 ant -25.45 -26.05 -25.67 -25.64 -25.50 -24.77 -26.64 -27.30 -29.03 -25.73 -23.57
23 fly -24.45 -24.90 -25.03 -25.45 -25.53 -24.33 -24.12 -24.99 -25.01 -24.39 -27.08 -28.99 -24.54 -25.17 -25.30 -24.45
24 pyr -24.54 -23.95 -25.26 -26.20 -25.45 -23.22 -23.66 -24.77 -24.99 -24.84 -27.32 -29.48 -24.91 -24.80 -24.84 -24.88
25 baa -23.81 -23.22 -24.29 -22.81 -24.69 -23.04 -23.00 -23.12 -23.76 -22.45 -26.60 -27.47 -23.54 -24.19 -23.98 -25.96
26 chr -24.80 -24.12 -25.18 -25.12 -25.74 -23.59 -24.27 -24.37 -24.95 -23.54 -26.80 -28.16 -25.12 -25.85 -25.29 -24.20
27,28 bbkf -24.45 -26.33 -26.17 -26.69 -26.14 -27.17 -24.34 -25.68
29 bap -24.63 -23.82 -25.68 -26.04 -26.24 -25.47 -24.64 -26.73 -26.66 -27.08 -27.17 -27.25 -26.13 -24.66
30,31 ip_dba -23.37 -25.27 -26.20 -26.88 -26.30 -24.72 -25.26 -23.07 -23.69 -24.03
32 bp -23.58 -24.48 -24.03 -22.14 -23.75 -25.17 -26.48 -27.87 -25.54 -26.08
PT PTP -24.44 -25.55 -26.05 -26.56 -26.49 -25.60 -25.60 -26.19 -27.39 -26.38 -25.96 -26.29 -26.38 -26.82 -26.33 -25.95
9D -31.53 -31.49 -31.42 -32.52 -31.38 -31.47 -31.52 -31.63 -31.07 -31.59 -31.13 -31.01 -31.84 -31.52 -31.18 -30.99
10D -32.74 -32.81 -32.80 -32.75 -32.59 -32.42 -32.89 -33.05 -32.69 -32.65 -32.33 -32.22 -33.03 -32.99 -32.52 -32.54
16D -30.89 -30.86 -31.30 -30.68 -31.30 -30.14 -29.98 -30.60 -31.06 -30.50 -31.16 -29.74 -30.33 -31.00 -30.87 -30.47
19D -27.27 -27.44 -27.03 -27.75 -26.60 -27.13 -26.97 -27.53 -27.14 -27.53 -27.00 -27.45 -27.40 -28.77 -27.55 -27.46
24D -26.83 -26.12 -26.69 -26.71 -26.69 -26.43 -26.37 -26.88 -26.98 -26.85 -26.53 -26.25 -26.68 -26.79 -26.73 -26.42
32D -29.52 -28.27 -29.44 -29.34 -28.32 -28.60 -29.35 -29.64 -29.84 -29.81 -29.44 -29.51 -29.95 -30.12 -29.84 -29.36
SS2 2FBP -30.89 -31.86 -31.30 -31.52 -31.32 -31.11 -30.76 -31.34 -31.71 -31.46 -31.08 -31.14 -31.28 -31.91 -31.39 -31.09
IS2 OTP -29.08
SS3 5AA -27.18 -28.27
PT PTP -24.44 -25.55 -26.01 -26.56 -26.49 -25.60 -25.60 -26.19 -27.39 -26.38 -25.96 -26.29 -26.38 -26.82 -26.33 -25.95
FS1 dbt -25.58
FS2 bn -25.08 -27.91 -25.04 -25.98 -25.99 -23.42 -24.26 -25.39 -24.87 -26.11 -24.05 -26.24 -25.90 -24.67 -24.95
FS3 cod -26.01 -28.7757142857
&L&P&R&F&A

ptp

T185
DA09
DA10
MT03
MT07
MT09
MT10
DA05
RF08
RF09
RF NAPL
MT NAPL
RF01
RF06
DA NAPL
PTP
Sample Name
isotope ratio
p-terphenyl
-24.44
-25.545
-26.01
-26.56
-26.4866666667
-25.6025
-25.595
-26.1866666667
-27.385
-26.375
-25.9633333333
-26.29
-26.38
-26.82
-26.33

24D

T185
DA09
DA10
MT03
MT07
MT09
MT10
DA05
RF08
RF09
RF NAPL
MT NAPL
RF01
RF06
DA NAPL
24D
Sample Name
Isotope Ratio
Internal Standard 24D
-26.8266666667
-26.12
-26.69
-26.705
-26.69
-26.4325
-26.365
-26.8766666667
-26.98
-26.85
-26.5333333333
-26.25
-26.68
-26.7933333333
-26.73

pyrene

T185
DA09
DA10
MT03
MT07
MT09
MT10
DA05
RF08
RF09
RF NAPL
MT NAPL
RF01
RF06
DA NAPL
pyr
sample name
δ13C (‰) pyrene
-24.5366666667
-23.95
-25.26
-26.2
-25.4544444444
-23.22
-23.66
-24.77
-24.985
-24.84
-27.3233333333
-29.48
-24.91
-24.8
-24.84

RF data

-27.17
-27.9433333333
-27.8533333333
-22.62 -24.73
-27.95
-23.6
-27.52 -22.46
-27.4633333333 -24.19 -24.04 -24.19
-27.2966666667 -26.64
-27.08 -25.01 -24.39 -24.54 -25.17
-27.3233333333 -24.985 -24.84 -24.91 -24.8
-26.5966666667 -23.76 -22.45 -23.54 -24.19
-26.8 -24.95 -23.54 -25.12 -25.85
-26.14 -26.69 -27.17
-27.08 -26.73 -26.66 -27.17 -27.25
-24.72 -26.3 -25.26 -23.07
-26.48 -23.75 -25.17 -27.87
RF NAPL
ILRF08
ILRF09
ILRF01
ILRF06
Compound
δ13C (‰)

Sheet1

δ13C (‰)

NAPLS

nap -27.17
mn2 -27.9433333333 -28.405 -24.5933333333
mn1 -27.8533333333 -28.0233333333 -24.5933333333
acy -22.62 -26.72 -23.9466666667
ace -27.95 -24.84
dbf -23.6 -26.08 -23.41
flu -27.52 -28.6166666667 -24.6766666667
phe -27.4633333333 -28.6 -24.6866666667
ant -27.2966666667 -29.0333333333 -25.73
fly -27.08 -28.99 -25.3033333333
pyr -27.3233333333 -29.48 -24.84
baa -26.5966666667 -27.4733333333 -23.98
chr -26.8 -28.1566666667 -25.2933333333
bbkf -26.14 -24.3366666667
bap -27.08 -26.13
ip_dba -24.72 -23.69
bp -26.48 -25.54
T185
RF NAPL
MT NAPL
DA NAPL
Compound
δ13C (‰)
-23.3
-23.42
-23.5866666667
-22.81
-22.63
-24.4366666667
-24.645
-25.4533333333
-24.45
-24.5366666667
-23.8133333333
-24.7966666667
-24.4533333333
-24.63
-23.37

MT

nap nap nap nap
mn2 mn2 mn2 mn2 -28.405
mn1 mn1 mn1 mn1 -28.0233333333
acy acy acy acy -26.72
ace ace ace ace
dbf dbf dbf dbf -26.08
flu flu flu flu -28.6166666667
phe phe phe phe -28.6
ant ant ant ant -29.0333333333
fly fly fly fly -28.99
pyr pyr pyr pyr -29.48
baa baa baa baa -27.4733333333
chr chr chr chr -28.1566666667
bbkf bbkf bbkf bbkf
bap bap bap bap
ip_dba ip_dba ip_dba ip_dba
bp bp bp bp
MT03
MT07
MT09
MT10
MT NAPL
Compound
δ13C (‰)
-25.63
-24.42
-24.19
-24.99
-23.98
-24.4
-24.28
-23.44
-24.03
-23.6
-24.53
-24.64
-23.65
-23.68
-25.64
-25.5
-25.45
-25.53
-24.33
-24.12
-26.2
-25.4544444444
-23.22
-23.66
-22.81
-24.69
-23.04
-23
-25.12
-25.74
-23.59
-24.27
-26.04
-26.24
-25.47
-24.64
-26.2
-24.03

double ratio

Sample fl/py ratio
T185 1.27 -24.54
ILRF08 1.52 -24.99
ILRF09 1.34 -24.84
ILRF01 1.2 -24.91
ILRF06 1.31 -24.8
RF NAPL 0.79 -27.32
Sample fl/py ratio
T185 1.27 -24.54
DA05 1.13 -24.77
DA09 1.15 -23.95
DA10 1.71 -25.26
DA NAPL 1.13 -24.84

double ratio

0
0
0
0
0
0
&L&F
T185
RF08
RF09
RF01
RF06
RF NAPL
fluoranthene/pyrene
δ13C (‰) pyrene
0
0
0
0
0
0
0
0
0
0
0
T185
0
0
0
0
0

PAH Fingerprints and Isotopes Show No MGP Contribution to Background

*

MT03, MT07, MT09, MT10 – background soil samples from town

MT NAPL – tar from MGP site in town

TPAH-ug/kg Fl/Py
MT03 2,510 1.177
MT07 11,100 1.136
MT09 3,880 1.194
MT10 6,940 1.208
MT NAPL 0.64

GC conditions

META PAH Samples 10 / 02

GC and column conditions :
Column: Restek RTX-5 / 30m, 0.25 dia., 0.50 um film
Column flow: 1.4 mls/min
Injector: 310 C / Splitless
GC program:
40C ( hold 1.5m) 4 deg. @min. to 300 ; hold 25 min. / 90 min. total

Carbon Combustion Interface: 940 C

lab dups MT07

nap nap nap nap
mn2 mn2 mn2 mn2
mn1 mn1 mn1 mn1
acy acy acy acy
ace ace ace ace
dba dba dba dba
flu flu flu flu
phe phe phe phe
ant ant ant ant
fly fly fly fly
pyr pyr pyr pyr
baa baa baa baa
chr chr chr chr
bbkf bbkf bbkf bbkf
bap bap bap bap
ip_dba ip_dba ip_dba ip_dba
bp bp bp bp
ILMT07
ILMT07 R1
ILMT07 R2
ILMT07 AVE
Compound
Isotope ratio
-25.63
-25.63
-25.43
-24.99
-27.42
-24.28
-26.69
-24.03
-23.97
-24.6666666667
-24.78
-24.64
-24.6955555556
-26.24
-25.46
-25.5
-25.7333333333
-25.67
-25.1633333333
-25.53
-25.4544444444
-25.6833333333
-25.16
-25.45
-25.4311111111
-24.27
-24.49
-24.69
-24.4833333333
-26.31
-24.9466666667
-25.74
-25.6655555556
-28.46
-27.78
-28.78
-28.34
-25.42
-26.19
-26.24
-26.03
-26.2
-27.76
-21.76
-23.76

PAH data

Average Average Average Average Average Average Average Average Average Average Average Average Average Average Average Average Average Average
Peak# Name OU 4007 Ical T-185 ER011212-02 DF ER011212-03AVE ER011212-07 DF ER011212-14 DF ER011212-14 DUP 1DF ER011212-14DUP 2DF ER011212-14AVE ER011212-16DF ER011212-17 DF ER011212-151 DF ER11115-02 DF ER11115-03 DF GT020702-01 DF GTO020719-03 DF ER020827-01 DUP 1DF ER020827-01 DUP 2DF ER020827-01AVE ER020827-02 DF GT020828-01 DF
ICAL T185
3 eb -28.41
4 m/p-x -27.00 -23.33
5,6 ox_sty -27.08 -23.61
7 ipt -27.08
8 pb -28.03
9 tmb135 -27.47
10 tmb124 -26.88 -20.54
11 sbb -23.47
12 pipt -32.83
13 nbb -25.96
14 nap -24.91 -24.24 -25.63 -25.63 -24.42 -27.17
15 mn2 -22.47 -23.30 -23.84 -25.43 -24.99 -27.42 -23.98 -24.40 -27.94 -28.41 -24.59
16 mn1 -30.25 -23.42 -24.18 -24.28 -26.69 -23.44 -27.85 -28.02 -24.59
17 acy -21.93 -23.59 -25.10 -24.73 -22.62 -26.72 -23.95
18 ace -24.04 -22.81 -24.08 -27.95 -24.84
19 dba -23.39 -22.63 -22.23 -24.03 -23.97 -23.60 -23.60 -26.08 -23.41
20 flu -24.23 -24.44 -23.51 -25.67 -22.46 -27.52 -28.62 -24.68
21 phe -23.37 -24.65 -24.09 -24.66 -24.67 -24.78 -24.64 -24.70 -23.65 -23.68 -24.45 -24.19 -24.04 -27.46 -28.60 -24.19 -24.69
22 ant -23.57 -25.45 -25.67 -26.24 -25.46 -25.50 -25.73 -24.77 -26.64 -27.30 -29.03 -25.93 -25.73
23 fly -24.45 -24.45 -24.90 -25.03 -26.20 -25.67 -25.16 -25.53 -25.45 -24.33 -24.12 -24.99 -25.01 -24.39 -27.08 -28.99 -24.91 -24.30 -24.60 -25.17 -25.30
24 pyr -24.88 -24.54 -23.95 -25.26 -24.07 -25.68 -25.16 -25.45 -25.43 -23.22 -23.66 -24.77 -24.99 -24.84 -27.32 -29.48 -24.71 -25.05 -24.84 -24.80 -24.84
25 baa -25.96 -23.81 -23.22 -24.29 -22.81 -24.27 -24.49 -24.69 -24.48 -23.04 -23.00 -23.12 -23.76 -22.45 -26.60 -27.47 -23.71 -23.43 -24.19 -23.98
26 chr -24.20 -24.80 -24.12 -25.18 -25.12 -26.31 -24.95 -25.74 -25.67 -23.59 -24.27 -24.37 -24.95 -23.54 -26.80 -28.16 -25.46 -24.90 -24.98 -25.85 -25.29
27,28 bbkf -25.68 -24.45 -26.33 -26.17 -33.41 -28.46 -27.78 -28.78 -28.34 -31.87 -28.27 -32.77 -29.69 -31.01 -26.14 -27.83 -28.61 -25.94 -31.83 -27.17 -24.34
29 bap -24.66 -24.63 -23.82 -25.68 -26.04 -25.42 -26.19 -26.24 -26.03 -25.47 -24.64 -29.92 -26.37 -26.66 -27.08 -29.13 -26.42 -27.42 -27.25 -26.13
30,31 ip_dba -24.03 -23.37 -29.67 -25.27 -26.20 -27.76 -26.88 -26.30 -24.72 -25.26 -30.63 -23.07 -23.69
32 bp -26.08 -26.22 -23.58 -24.48 -20.81 -21.76 -23.76 -22.59 -24.03 -22.14 -23.75 -25.17 -26.48 -22.68 -21.42 -20.60 -27.87 -25.54
9D -30.99 -31.53 -31.49 -31.42 -32.52 -31.21 -31.68 -31.38 -31.42 -31.47 -31.52 -31.63 -31.07 -31.59 -31.13 -31.01 -31.30 -32.21 -31.75 -31.52 -31.18
10D -32.54 -32.74 -32.81 -32.80 -32.75 -32.63 -33.24 -32.59 -32.82 -32.42 -32.89 -33.05 -32.69 -32.65 -32.33 -32.22 -32.47 -33.41 -32.94 -32.99 -32.52
16D -30.47 -30.89 -30.86 -30.46 -30.68 -30.62 -30.62 -30.76 -30.67 -30.14 -29.98 -30.60 -31.06 -30.50 -31.16 -29.74 -30.37 -30.31 -30.34 -31.00 -30.87
19D -27.46 -27.27 -27.44 -27.03 -27.75 -27.40 -27.37 -27.24 -27.34 -27.13 -26.97 -27.53 -27.14 -27.53 -27.00 -27.45 -27.32 -27.46 -27.39 -28.77 -27.55
24D -26.42 -26.83 -26.12 -26.69 -26.71 -26.68 -26.94 -26.82 -26.81 -26.43 -26.37 -26.88 -26.98 -26.85 -26.53 -26.25 -26.58 -26.75 -26.66 -26.79 -26.73
32D -29.36 -29.52 -28.27 -29.44 -29.34 -29.77 -29.10 -29.35 -29.41 -28.60 -29.35 -29.64 -29.84 -29.81 -29.44 -29.51 -29.65 -30.15 -29.90 -30.12 -29.84
SS2 2FBP -31.09 -30.89 -31.86 -31.30 -31.52 -31.60 -31.50 -31.43 -31.51 -31.11 -30.76 -31.34 -31.71 -31.46 -31.08 -31.14 -31.13 -31.33 -31.23 -31.91 -31.39
IS2 OTP -29.08
SS3 5AA -28.27 -27.18
PT PTP -25.95 -24.44 -25.55 -26.05 -26.56 -26.67 -26.34 -26.49 -26.50 -25.60 -25.60 -26.19 -27.39 -26.38 -25.96 -26.69 -26.24 -26.47 -26.36 -26.82 -26.33
FS1 dbt -25.58 -24.45
FS2 bn -24.95 -25.08 -27.91 -25.04 -25.98 -24.88 -26.90 -25.99 -25.92 -23.42 -24.26 -25.39 -24.87 -26.11 -24.05 -27.50 -25.40 -26.45 -25.90 -24.67
FS3 cod -28.78 -25.52 -27.25 -26.01 -26.81
&L&P&R&F&A

Chart1

-30.89
-30.86
-31.3
-30.675
-31.3
-30.1433333333
-29.98
-30.6033333333
-31.055
-30.5
-31.1633333333
-29.7366666667
-30.33
-31.0033333333
-30.87
-30.4671428571
16D

DA

nap nap nap nap
mn2 mn2 mn2 mn2
mn1 mn1 mn1 mn1
acy acy acy acy
ace ace ace ace
dbf dbf dbf dbf
flu flu flu flu
phe phe phe phe
ant ant ant ant
fly fly fly fly
pyr pyr pyr pyr
baa baa baa baa
chr chr chr chr
bbkf bbkf bbkf bbkf
bap bap bap bap
ip_dba ip_dba ip_dba ip_dba
bp bp bp bp
DA10
DA09
DA05
DA NAPL
Compound
δ13C (‰)
-24.24
-23.84
-24.5933333333
-24.18
-24.5933333333
-25.1
-23.9466666667
-24.08
-24.84
-22.23
-23.41
-23.51
-25.67
-24.6766666667
-24.06
-24.09
-24.45
-24.6866666667
-25.67
-26.05
-24.77
-25.73
-25.03
-24.9
-24.99
-25.3033333333
-25.26
-23.95
-24.77
-24.84
-24.29
-23.22
-23.12
-23.98
-25.18
-24.12
-24.37
-25.2933333333
-26.17
-26.33
-24.3366666667
-25.68
-23.815
-26.13
-25.27
-26.88
-23.69
-24.48
-23.58
-22.14
-25.54

PAH data (2)

Average Average Average Average Average Average Average Average Average Average Average Average Average
Peak# Name T-185 ER011212-02 DF ER011212-03AVE ER011212-07 DF ER011212-14AVE ER011212-16DF ER011212-17 DF ER011212-151 DF ER11115-02 DF ER11115-03 DF GT020702-01 DF GTO020719-03 DF ER020827-01AVE ER020827-02 DF GT020828-01 DF OU 4007 Ical
T185 DA09 DA10 MT03 MT07 MT09 MT10 DA05 RF08 RF09 RF NAPL MT NAPL RF01 RF06 DA NAPL ICAL
3 eb -28.41
4 m/p-x -23.33 -27.00
5,6 ox_sty -23.61 -27.08
7 ipt -27.08
8 pb -28.03
9 tmb135 -27.47
10 tmb124 -20.54 -26.88
11 sbb -23.47
12 pipt -32.83
13 nbb -25.96
14 nap -24.24 -25.63 -24.42 -24.19 -27.17 -24.91
15 mn2 -23.30 -23.84 -24.99 -23.98 -24.40 -27.94 -28.41 -24.59 -22.47
16 mn1 -23.42 -24.18 -24.28 -23.44 -27.85 -28.02 -24.59 -30.25
17 acy -23.59 -25.10 -24.73 -22.62 -26.72 -23.95 -21.93
18 ace -22.81 -24.08 -27.95 -24.84 -24.04
19 dbf -22.63 -22.23 -24.03 -23.60 -23.60 -26.08 -23.41 -23.39
20 flu -24.44 -23.51 -25.67 -22.46 -27.52 -28.62 -24.68 -24.23
21 phe -24.65 -24.09 -24.06 -24.53 -24.64 -23.65 -23.68 -24.45 -24.19 -24.04 -27.46 -28.60 -24.19 -24.69 -23.37
22 ant -25.45 -26.05 -25.67 -25.64 -25.50 -24.77 -26.64 -27.30 -29.03 -25.73 -23.57
23 fly -24.45 -24.90 -25.03 -25.45 -25.53 -24.33 -24.12 -24.99 -25.01 -24.39 -27.08 -28.99 -24.54 -25.17 -25.30 -24.45
24 pyr -24.54 -23.95 -25.26 -26.20 -25.45 -23.22 -23.66 -24.77 -24.99 -24.84 -27.32 -29.48 -24.91 -24.80 -24.84 -24.88
25 baa -23.81 -23.22 -24.29 -22.81 -24.69 -23.04 -23.00 -23.12 -23.76 -22.45 -26.60 -27.47 -23.54 -24.19 -23.98 -25.96
26 chr -24.80 -24.12 -25.18 -25.12 -25.74 -23.59 -24.27 -24.37 -24.95 -23.54 -26.80 -28.16 -25.12 -25.85 -25.29 -24.20
27,28 bbkf -24.45 -26.33 -26.17 -26.69 -26.14 -27.17 -24.34 -25.68
29 bap -24.63 -23.82 -25.68 -26.04 -26.24 -25.47 -24.64 -26.73 -26.66 -27.08 -27.17 -27.25 -26.13 -24.66
30,31 ip_dba -23.37 -25.27 -26.20 -26.88 -26.30 -24.72 -25.26 -23.07 -23.69 -24.03
32 bp -23.58 -24.48 -24.03 -22.14 -23.75 -25.17 -26.48 -27.87 -25.54 -26.08
PT PTP -24.44 -25.55 -26.05 -26.56 -26.49 -25.60 -25.60 -26.19 -27.39 -26.38 -25.96 -26.29 -26.38 -26.82 -26.33 -25.95
9D -31.53 -31.49 -31.42 -32.52 -31.38 -31.47 -31.52 -31.63 -31.07 -31.59 -31.13 -31.01 -31.84 -31.52 -31.18 -30.99
10D -32.74 -32.81 -32.80 -32.75 -32.59 -32.42 -32.89 -33.05 -32.69 -32.65 -32.33 -32.22 -33.03 -32.99 -32.52 -32.54
16D -30.89 -30.86 -31.30 -30.68 -31.30 -30.14 -29.98 -30.60 -31.06 -30.50 -31.16 -29.74 -30.33 -31.00 -30.87 -30.47
19D -27.27 -27.44 -27.03 -27.75 -26.60 -27.13 -26.97 -27.53 -27.14 -27.53 -27.00 -27.45 -27.40 -28.77 -27.55 -27.46
24D -26.83 -26.12 -26.69 -26.71 -26.69 -26.43 -26.37 -26.88 -26.98 -26.85 -26.53 -26.25 -26.68 -26.79 -26.73 -26.42
32D -29.52 -28.27 -29.44 -29.34 -28.32 -28.60 -29.35 -29.64 -29.84 -29.81 -29.44 -29.51 -29.95 -30.12 -29.84 -29.36
SS2 2FBP -30.89 -31.86 -31.30 -31.52 -31.32 -31.11 -30.76 -31.34 -31.71 -31.46 -31.08 -31.14 -31.28 -31.91 -31.39 -31.09
IS2 OTP -29.08
SS3 5AA -27.18 -28.27
PT PTP -24.44 -25.55 -26.01 -26.56 -26.49 -25.60 -25.60 -26.19 -27.39 -26.38 -25.96 -26.29 -26.38 -26.82 -26.33 -25.95
FS1 dbt -25.58
FS2 bn -25.08 -27.91 -25.04 -25.98 -25.99 -23.42 -24.26 -25.39 -24.87 -26.11 -24.05 -26.24 -25.90 -24.67 -24.95
FS3 cod -26.01 -28.7757142857
&L&P&R&F&A

ptp

T185
DA09
DA10
MT03
MT07
MT09
MT10
DA05
RF08
RF09
RF NAPL
MT NAPL
RF01
RF06
DA NAPL
PTP
Sample Name
isotope ratio
p-terphenyl
-24.44
-25.545
-26.01
-26.56
-26.4866666667
-25.6025
-25.595
-26.1866666667
-27.385
-26.375
-25.9633333333
-26.29
-26.38
-26.82
-26.33

24D

T185
DA09
DA10
MT03
MT07
MT09
MT10
DA05
RF08
RF09
RF NAPL
MT NAPL
RF01
RF06
DA NAPL
24D
Sample Name
Isotope Ratio
Internal Standard 24D
-26.8266666667
-26.12
-26.69
-26.705
-26.69
-26.4325
-26.365
-26.8766666667
-26.98
-26.85
-26.5333333333
-26.25
-26.68
-26.7933333333
-26.73

pyrene

T185
DA09
DA10
MT03
MT07
MT09
MT10
DA05
RF08
RF09
RF NAPL
MT NAPL
RF01
RF06
DA NAPL
pyr
sample name
δ13C (‰) pyrene
-24.5366666667
-23.95
-25.26
-26.2
-25.4544444444
-23.22
-23.66
-24.77
-24.985
-24.84
-27.3233333333
-29.48
-24.91
-24.8
-24.84

RF data

-27.17
-27.9433333333
-27.8533333333
-22.62 -24.73
-27.95
-23.6
-27.52 -22.46
-27.4633333333 -24.19 -24.04 -24.19
-27.2966666667 -26.64
-27.08 -25.01 -24.39 -24.54 -25.17
-27.3233333333 -24.985 -24.84 -24.91 -24.8
-26.5966666667 -23.76 -22.45 -23.54 -24.19
-26.8 -24.95 -23.54 -25.12 -25.85
-26.14 -26.69 -27.17
-27.08 -26.73 -26.66 -27.17 -27.25
-24.72 -26.3 -25.26 -23.07
-26.48 -23.75 -25.17 -27.87
RF NAPL
ILRF08
ILRF09
ILRF01
ILRF06
Compound
δ13C (‰)

Sheet1

δ13C (‰)

NAPLS

nap -27.17
mn2 -27.9433333333 -28.405 -24.5933333333
mn1 -27.8533333333 -28.0233333333 -24.5933333333
acy -22.62 -26.72 -23.9466666667
ace -27.95 -24.84
dbf -23.6 -26.08 -23.41
flu -27.52 -28.6166666667 -24.6766666667
phe -27.4633333333 -28.6 -24.6866666667
ant -27.2966666667 -29.0333333333 -25.73
fly -27.08 -28.99 -25.3033333333
pyr -27.3233333333 -29.48 -24.84
baa -26.5966666667 -27.4733333333 -23.98
chr -26.8 -28.1566666667 -25.2933333333
bbkf -26.14 -24.3366666667
bap -27.08 -26.13
ip_dba -24.72 -23.69
bp -26.48 -25.54
T185
RF NAPL
MT NAPL
DA NAPL
Compound
δ13C (‰)
-23.3
-23.42
-23.5866666667
-22.81
-22.63
-24.4366666667
-24.645
-25.4533333333
-24.45
-24.5366666667
-23.8133333333
-24.7966666667
-24.4533333333
-24.63
-23.37

MT

nap nap nap nap
mn2 mn2 mn2 mn2 -28.405
mn1 mn1 mn1 mn1 -28.0233333333
acy acy acy acy -26.72
ace ace ace ace
dbf dbf dbf dbf -26.08
flu flu flu flu -28.6166666667
phe phe phe phe -28.6
ant ant ant ant -29.0333333333
fly fly fly fly -28.99
pyr pyr pyr pyr -29.48
baa baa baa baa -27.4733333333
chr chr chr chr -28.1566666667
bbkf bbkf bbkf bbkf
bap bap bap bap
ip_dba ip_dba ip_dba ip_dba
bp bp bp bp
MT03
MT07
MT09
MT10
MT NAPL
Compound
δ13C (‰)
-25.63
-24.42
-24.19
-24.99
-23.98
-24.4
-24.28
-23.44
-24.03
-23.6
-24.53
-24.64
-23.65
-23.68
-25.64
-25.5
-25.45
-25.53
-24.33
-24.12
-26.2
-25.4544444444
-23.22
-23.66
-22.81
-24.69
-23.04
-23
-25.12
-25.74
-23.59
-24.27
-26.04
-26.24
-25.47
-24.64
-26.2
-24.03

double ratio

Sample fl/py ratio
T185 1.27 -24.54
ILRF08 1.52 -24.99
ILRF09 1.34 -24.84
ILRF01 1.2 -24.91
ILRF06 1.31 -24.8
RF NAPL 0.79 -27.32
Sample fl/py ratio
T185 1.27 -24.54
DA05 1.13 -24.77
DA09 1.15 -23.95
DA10 1.71 -25.26
DA NAPL 1.13 -24.84

double ratio

0
0
0
0
0
0
&L&F
T185
RF08
RF09
RF01
RF06
RF NAPL
fluoranthene/pyrene
δ13C (‰) pyrene
0
0
0
0
0
0
0
0
0
0
0
T185
0
0
0
0
0

Summary

  • Environmental Forensics combines a variety of analytical tools to typically provide information on origin and state of contaminants in the environment.
  • One of these tools involves utilization of stable isotopes.
  • In some situations stable isotope data compliments other analytical data. In other cases may be only tool available.

-140

-136

-132

-128

-124

-120

-29-28-27-26-25-24-23

R

-32

-30

-28

-26

-24

-22

-20

C14iC15iC16iC18iPRPH

Carbon Isotope Value

-27

-26

-25

-24

-23

-22

-21

-20

C14iC15iC16iC18iPRPH

MW 6

MW 1

-35.00

-33.00

-31.00

-29.00

-27.00

-25.00

-23.00

-21.00

1416182022242629329D16D24D

Peak ID

Carbon isotope value

7

8

-38.00

-36.00

-34.00

-32.00

-30.00

-28.00

-26.00

-24.00

-22.00

-20.00

141720232630,9D19D

Peak ID

C Isotope value

02DF

08DF

-30.00

-29.00

-28.00

-27.00

-26.00

-25.00

-24.00

-23.00

-22.00

nap

mn2mn1

acy

ace

dbf

flu

phe

ant

fly

pyr

baa

chr

bbkf

bap

ip_dba

bp

Compound

δ13C (‰)

T185

RF NAPL

MT NAPL

DA NAPL

-30.00

-29.00

-28.00

-27.00

-26.00

-25.00

-24.00

-23.00

-22.00

nap

mn2mn1

acyace

dbf

flu

phe

ant

fly

pyr

baa

chr

bbkf

bap

ip_dba

bp

Compound

δ13C (‰)

MT03

MT07

MT09

MT10

MT NAPL